FEB 14, 2017 04:17 PM PST

Promising New Technique Uses Stem Cells to fix Bone Damage

WRITTEN BY: Carmen Leitch
2 14 509

New work has demonstrated that bone injuries can be repaired using a sheet of a carbon compound that has photocatalytic characteristics, and combining it with stem cells derived from human bone marrow. These developments could lead to new treatments that can exert a powerful positive effect on skeletal fractures or periodontal disease. Check out the short video below that summarizes the findings in the report, which was published in the journal ACS Nano.

 

The scientists found that a chemical sheet made of carbon nitride, which absorbs red light, can support the growth of bone cells - osteogenic differentiation -  because of the activation of a molecule that promotes gene expression. That molecule is a transcription factor called runt-related transcription factor 2 or Runx2. The absorption of red light and the emission of fluorescence by the carbon nitride sheets accelerates bone regeneration. It seems that when the sheets are exposed to red light in liquid conditions where cells are growing, electrons are released, which stimulates the accumulation of calcium in cell cytoplasm.

These carbon nitrogen derivatives were made by the scientists using melamine chemical compounds, a type of plastic material. An examination of this mechanism and repeated testing did not reveal any kind of toxicity to either healthy or cancer cells when they were grown on the sheets in culture, indicating that these carbon nitride sheets have the potential for use as a biomaterial. 

The researchers also found that the sheets stimulate the formation of minerals by making stem cells turn into or differentiate into bone cells. Aside from Runx2, other genes that indicate bone cell differentiation, osteogenic differentiation markers ALP, OCN and BSP, were found to be more highly expressed.

A team of scientists from UNIST conducted this study, including members Young-Kyo Seo, a Professor of Life Sciences, Professor of Chemistry Dr. Jitendra N. Tiwari, Professor of Natural Sciences Kwang S. Kim, and Professor of Life Sciences Pann-Ghill Suh, as well as several other researchers.

"This research has opened up the possibility of developing a new medicine that effectively treats skeletal injuries, such as fractures and osteoporosis," commented Professor Young-Kyo Seo. "It will be a very useful tool for making artificial joints and teeth with the use of 3D printing."

(Upper left) Chemical bonding and physical structure of carbon nitride sheets. (Lower left) In a liquid state, red light is transmitted at a maximum of 450nm and emitted at a wavelength of 635 nm. (Right) After four weeks of loading carbon nitride sheets into the skull-damaged mice, the skull was regenerated by more than 90 percent. / Credit: UNIST

"This is an important milestone in the analysis of biomechanical functions needed for the development of biomaterials, including adjuvants for hard tissues such as damaged bones and teeth," he concluded. 

While a lot more work is still needed before the clinical applications can be realized, the researchers believe their work has opened up myriad possibilities for using carbon nitride sheets in future bone therapeutics. 

 

Sources: AAAS/Eurekalert! via UNIST, ACS Nano

About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
JUN 15, 2018
Genetics & Genomics
JUN 15, 2018
Why Blood Cells Originate in Bone
Adults typically produce billions of new blood cells every day.
JUN 21, 2018
Immunology
JUN 21, 2018
The Silver Tsunami: An Aging Immune System and Cancer
Why do cases of cancer become more common as we get older? Scientists interested in explaining the so-called “Silver Tsunami” phenomenon look t
JUN 27, 2018
Immunology
JUN 27, 2018
Immune Cells Responsible for Chemo-induced Diarrhea
While studying specific immune cells in the context of chronic itching in the skin, two Washington University School of Medicine scientists discovered that
JUN 28, 2018
Immunology
JUN 28, 2018
Synthetic T Cells the Next Big Thing in Immunotherapy
The highly complex structure and function of human T cells made the creation of synthetic versions of the immune cells difficult, but scientists from Unive
JUL 30, 2018
Cell & Molecular Biology
JUL 30, 2018
A Complete View of the Fly Brain at Nanoscale Resolution
Researchers have completed a massive project to create a high-resolution map of the adult fruit fly brain.
AUG 06, 2018
Cell & Molecular Biology
AUG 06, 2018
Learning More About Addiction Relapse
Researchers studying cocaine addiction have managed to significantly reduce relapse rates in a preclinical model.
Loading Comments...