SEP 12, 2018 5:33 PM PDT

Tightening Control of Bacterial Gene Expression

WRITTEN BY: Carmen Leitch

Bacteria are easy to work with; their genes can be manipulated, and it’s usually easy to grow a lot of them. As such, they have a lot of potential as mini-factories that could produce many kinds of chemicals, from therapeutics to fuels. But one hurdle has been finding an affordable way to get the expression of those bacterial genes under control. To overcome that challenge, scientists from the Department of Energy's (DOE) Joint BioEnergy Institute, Lawrence Livermore National Laboratory (LLNL), Lawrence Berkeley National Lab and San Francisco State University have created a system to control gene expression; they called it "Jungle Express." This work has been reported in Nature Communications.

Colorized scanning electron micrograph of Escherichia coli / Image credit: NIAID

"We have created a system that can precisely control the timing and levels of expression of the genes involved in these processes, opening the way for efficient large-scale production at low costs," said LLNL biochemist Michael Thelen, senior author of the paper. "These enhancements to bacterial gene regulation have a potential translation to sustainable bioproduction."

Being able to turn genes on and off is fundamental to research and industry. While there have been ways to do this, they had issues. One difficulty was so-called leaky gene expression; there isn’t tight control of when genes are on or off, and improper regulation meant targets get made incorrectly or at the wrong levels. It was also an expensive problem.

"The key component of this expression system is a DNA binding protein that acts as a gene regulator," said Basel University graduate student Thomas Ruegg, who worked with Helen. "We used a computational approach to predict a small DNA sequence for optimal binding of the regulator protein."

Crystal violet is a common, inexpensive dye. Ruegg found that the dye quickly causes the gene regulator to release, turning gene expression on and generating biochemicals. "In a rational approach, we used the optimized DNA binding site to engineer a robust system in which crystal violet acts as the inducer to trigger efficient gene expression," Ruegg explained.

"Our findings have the potential to overcome the bottlenecks encountered in earlier systems and opens the way for tightly controlled and efficient gene expression that is not restricted to host organism, substrate or scale," Thelen added.

The name the team used, Jungle Express, comes from a rainforest bacterium, which is where the regulator protein from this work is found in nature. Ruegg noted that "it is a fascinating journey to explore the inexhaustible resource of genetic information found in microorganisms, and to decouple it from its native context for the development of novel, useful applications."

Learn more about how bacteria might be used to make valuable compounds from the video; EMBL discusses how to engineer a microbe that produces bioplastic.

 

Sources: Phys.org via LLNL, Nature Communications

About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
MAR 04, 2020
Genetics & Genomics
MAR 04, 2020
DNA Fragments and Cartilage Recovered From 75-Million-Year-Old Dinosaur Bones
An international team of researchers has analyzed cartilage from a baby duckbilled dinosaur, and they have identified bi ...
MAR 28, 2020
Cancer
MAR 28, 2020
The Not So Minor Genetic Players in Head and Neck Cancers
Head and neck squamous cell carcinomas (HNSCC) are among the most prevalent cancers among smokers, linked to alcohol con ...
APR 04, 2020
Cannabis Sciences
APR 04, 2020
Only 50% Cannabis Genome Has Been Mapped
As more and more jurisdictions are decriminalizing cannabis usage, its popularity both for medicinal and recreational us ...
APR 05, 2020
Genetics & Genomics
APR 05, 2020
Gene Variants Impact Natural Sunscreen & Vitamin D Levels
Vitamin D deficiencies have been linked to a variety of chronic diseases including bone disorders and heart disease.
APR 23, 2020
Cell & Molecular Biology
APR 23, 2020
Do Organisms Have to Pass Down More Than Just DNA?
A new theory of heredity is proposed, which takes into account more than just genes in a genome.
APR 26, 2020
Genetics & Genomics
APR 26, 2020
Does Poor Sleep Lead to Obesity, or is the Opposite True?
For many years, researchers have been aware of the link between obesity and poor sleep or a lack of sleep. But what come ...
Loading Comments...