SEP 18, 2019 11:51 AM PDT

Detecting DNA - Without Amplification

WRITTEN BY: Carmen Leitch

As genetic technologies rapidly advance, totally new approaches are now possible in biomedical research. One innovation is the CRISPR-Chip. The CRISPR reagents include a guide RNA molecule that can target very small, specific parts of the genome. By immobilizing the CRISPR molecules onto the surface of graphene-based transistors, they can search for a target sequence in a sample. The chip can thus detect DNA sequences digitally, without amplification, by the programming the chip with a guide RNA. If the target sequence is bound, the conductivity of the graphene changes, which can be detected.

Earlier this year, the new tool was reported in Nature Biomedical Engineering and is described in the video. "The innovation is to bring the two together as a capture mechanism. "Our system does not use amplification; instead, it relies on CRISPR's genome-searching capability and graphene's sensitivity," explained Dr. Kiana Aran, who co-founded the company Nanosens.

CRISPR-Chip was made possible by integrating the CRISPR technology of Nanosens and the company Cardea’s handheld reader, which detects changes in the graphene-based transistor. They are now combining the companies, and are aiming to bring the product to market soon. It is called the Genome Sensor.

“To show the world how aligned and well-integrated the two teams already are from having worked closely together for so long, we decided to move up and launch our Early Access Program for the Genome Sensor today at AGBT, on the very day of the announcement of the proposed combination with of the two companies,” said Cardea CEO and Nanosens co-founder) Michael Heltzen.

The combined company is planning to introduce more CRISPR-Chip tools in the future. “The CRISPR-Chip has generated impressive levels of interest from companies both large and small, who realize the disruptive potential CRISPR-Chip has across many markets.  With our Innovation Partnership Program, Cardea can finally offer the combined solution and work as one big team,” said Dr. Francie Barron, Vice President of Cardea’s Innovation Partnership Program.

“In today’s society, we can get almost any information we need immediately via our smartphones and the internet, but really important Precision Health questions still take days or weeks,” noted Heltzen. “Cardea’s long term mission is to provide those answers immediately via FDA approved systems, and with the launch of our Early Access Program we’ve taken a key step towards realizing that vision.”

The alpha version of the Nanosens Genome Sensor, a handheld DNA search engine Credit: Courtesy of Cardea Bio

“We’re excited to become part of Cardea and develop new and innovative uses for our combined technologies.  To help realize the potential, we’re calling all genomics innovators to join the Early Access Program to accelerate our research and product development so that we can empower everyone with immediate insight into their genome via “Powered by Cardea” instruments,” said Dr. Aran, who will now be Chief Science Officer at Cardea. Learn more about joining the Early Access Program here.

About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
MAR 23, 2020
Cell & Molecular Biology
MAR 23, 2020
How a Father's Diet Can Impact the Health of His Offspring
When fathers consume a diet high in fat or low in protein it can increase the risk of metabolic disorders like diabetes ...
APR 29, 2020
Genetics & Genomics
APR 29, 2020
Toxoplasma Infections Can Cause Epigenetic Changes in Males
Anywhere from 25 to 80% of the world's population is infected with a parasite called Toxoplasma gondii.
MAY 15, 2020
Genetics & Genomics
MAY 15, 2020
It Only Takes One Gene For Virgin Birth in Honey Bees
Cape honey bees are found in South Africa, and while they look similar, they are very different from other subspecies of ...
MAY 19, 2020
Cancer
MAY 19, 2020
Great ape genomes closer to human tumors than human genomes
A new study analyzing cancer from an evolutionary perspective reports that the distribution of mutations in human tumors ...
MAY 24, 2020
Genetics & Genomics
MAY 24, 2020
How CRISPR Can Aid in Wildlife Conservation
Since it was developed, researchers have modified and applied the CRISPR gene editing technology in many different ways.
MAY 31, 2020
Genetics & Genomics
MAY 31, 2020
Regulating Gene Expression with Electricity
Scientists have created many different implantable devices and medicines that are as simple as pacemakers or as complex ...
Loading Comments...