SEP 28, 2016 09:38 AM PDT

News from the Primordial World

Image Credit: Noel Clark, University of Colorado

LA JOLLA, CA – September 28, 2016 – A new study led by scientists at The Scripps Research Institute (TSRI) offers a twist on a popular theory for how life on Earth began about four billion years ago.

The study questions the “RNA world” hypothesis, a theory for how RNA molecules evolved to create proteins and DNA. Instead, the new research offers evidence for a world where RNA and DNA evolved simultaneously.

“Even if you believe in a RNA-only world, you have to believe in something that existed with RNA to help it move forward,” said Ramanarayanan Krishnamurthy, associate professor of chemistry at TSRI and senior author of the new study. “Why not think of RNA and DNA rising together, rather than trying to convert RNA to DNA by means of some fantastic chemistry at a prebiotic stage?”

The study was published recently in the journal Angewandte Chemie.
 

A Look Back in Time


Researchers have explored the RNA world hypothesis for more than 30 years. The idea behind this theory is that a series of chemical reactions led to the formation of self-replicating RNA molecules. RNA then evolved to create proteins and enzymes that resembled early versions of what makes up life today. Eventually, these enzymes helped RNA produce DNA, which led to complex organisms.

On the surface, RNA and DNA molecules look similar, with DNA forming a ladder-like structure (with nucleobase pairs as the rungs and sugar molecule backbones as the sides) and RNA forming what looks like just one side of a ladder.

If the RNA world theory is accurate, some researchers believe there would have been many cases where RNA nucleotides were mixed with DNA backbones, creating “heterogeneous” strands. If stable, these blended “chimeras” would have been an intermediate step in the transition to DNA.
 

Problems with Instability


However, the new study shows a significant loss of stability when RNA and DNA share the same backbone. The chimeras do not stay together as well as pure RNA or pure DNA, which would compromise their ability to hold genetic information and replicate.

“We were surprised to see a very deep drop in what we would call the ‘thermal stability,’” said Krishnamurthy, who in addition to his position at TSRI has joint appointments with the National Science Foundation (NSF)-National Aeronautics and Space Administration (NASA) Center for Chemical Evolution and the Simons Collaboration on the Origins of Life. This instability appeared to be due to a difference in the DNA sugar molecule structure versus the RNA sugar molecule.

The finding supported previous research from Nobel laureate and Harvard University Chemistry and Chemical Biology Professor Jack Szostak that showed a loss of (nucleotide-binding aptamer) function when RNA mixed with DNA.

Because of this instability, chimeras in the RNA world would have likely died off in favor of more stable RNA molecules. This reflects what scientists see in cells today: If RNA nucleobases mistakenly join a DNA strand, sophisticated enzymes will rush to fix the mistake. Evolution has led to a system that favors more stable, “homogeneous” molecules.

These sophisticated enzymes were probably not around at the time of RNA and DNA’s early evolution, so these substitutions may have had a crippling effect on the molecules’ ability to replicate and function. “The transition from RNA to DNA would not have been easy without mechanisms to keep them separate,” said Krishnamurthy.
 

Considering a Second Theory


This realization led the scientists to consider an alternate theory: RNA and DNA may have arisen in tandem.

Krishnamurthy emphasized that his lab is not the first to propose this theory, but the findings on chimeric instability give scientists new evidence to consider.

If the two evolved at the same time, DNA could have established its own homogeneous system early on. RNA could have still evolved to produce DNA, but that may have occurred after it first met DNA and got to know its raw materials.

Krishnamurthy added that scientists will never know exactly how life began (barring the invention of a time machine), but by considering circumstances of early evolution, scientists can gain insights into the fundamentals of biology.

In addition to Krishnamurthy, authors of the study “RNA-DNA Chimeras in the Context of an RNA-world Transition to an RNA/DNA-world,” were Jesse V. Gavette (first author) and Matthias Stoop of TSRI and the NSF-NASA Center for Chemical Evolution; and Nicholas V. Hud of the Georgia Institute of Technology and the NSF-NASA Center for Chemical Evolution.

The study was supported by supported by the NSF and the NASA Exobiology Program, under the NSF-Center for Chemical Evolution (grant CHE-1504217).

This article was originally published on Scripps.edu.

TSRI Scientists Find Evidence for Alternate Theory of How Life Arose
About the Author
  • The Scripps Research Institute (TSRI) is one of the world's largest independent, not-for-profit organizations focusing on research in the biomedical sciences. TSRI is internationally recognized for its contributions to science and health, including its role in laying the foundation for new treatments for cancer, rheumatoid arthritis, hemophilia, and other diseases. An institution that evolved from the Scripps Metabolic Clinic founded by philanthropist Ellen Browning Scripps in 1924, the institute now employs about 2,700 people on its campuses in La Jolla, CA, and Jupiter, FL, where its renowned scientists-including two Nobel laureates-work toward their next discoveries. The institute's graduate program, which awards PhD degrees in biology and chemistry, ranks among the top ten of its kind in the nation. For more information, see www.scripps.edu.
You May Also Like
NOV 21, 2018
Cell & Molecular Biology
NOV 21, 2018
Preventing the Wrong Cells From Forming in Organoids
Organoids are advancing research by providing scientists with a 3D model of a human organ. But are they what they seem?...
NOV 23, 2018
Microbiology
NOV 23, 2018
Researchers Surprised to Find Giant Viruses in Forest Soil
Viruses were thought of as tiny infectious agents for the most part, until researchers began to discover more and more giant viruses....
NOV 24, 2018
Videos
NOV 24, 2018
Bats may Hold the Secret to Longevity
Some bats can live for up to 41 years, and many have a very healthy life....
DEC 26, 2018
Immunology
DEC 26, 2018
When the Immune System Makes a Mistake: Autoimmunity
A team from Scripps Research has found a molecular cause of a group of rare autoimmune disorders in which the immune system attacks the body's healthy cells....
DEC 29, 2018
Genetics & Genomics
DEC 29, 2018
Kidney Disease Risk Variant Found in New Populations
While we all carry the same genes in our genome, small variations within those genes can cause huge biological changes....
JAN 09, 2019
Cell & Molecular Biology
JAN 09, 2019
New Findings on the Early Stages of Autism Spectrum Disorder
While the prevalence of autism spectrum disorder has grown, there is still a lot we don't know about what causes the disorder....
Loading Comments...