A first-of-its-kind study shows that who we inherit genetic variants from - our mother or father - is crucial for the development of diseases and for research studies aimed at finding causes and potential treatments.
You might resemble or act more like your mother, but a novel research study from UNC School of Medicine researchers reveals that mammals are genetically more like their fathers. Specifically, the research shows that although we inherit equal amounts of genetic mutations from our parents - the mutations that make us who we are instead of some other person - we actually "use" more of the DNA that we inherit from our dads.
The research, published in the journal Nature Genetics, has wide implications for the study of human disease, especially when using mammalian research models. For instance, in many mouse models created for the study of gene expression related to disease, researchers typically don't take into account whether specific genetic expression originates from mothers or fathers. But the UNC research shows that inheriting a mutation has different consequences in mammals, depending on whether the genetic variant is inherited from the mother or father.
"This is an exceptional new research finding that opens the door to an entirely new area of exploration in human genetics," says Fernando Pardo-Manuel de Villena, PhD, professor of genetics and senior author of the paper. "We've known that there are 95 genes that are subject to this parent-of-origin effect. They're called imprinted genes, and they can play roles in diseases, depending on whether the genetic mutation came from the father or the mother. Now we've found that in addition to them, there are thousands of other genes that have a novel parent-of-origin effect."
These genetic mutations that are handed down from parents show up in many common but complex diseases that involve many genes, such as type-2 diabetes, heart disease, schizophrenia, obesity, and cancers. Studying them in genetically diverse mouse models that take parent-of-origin into account will give scientists more precise insights into the underlying causes of disease and the creation of therapeutics or other interventions.
The key to this research is the Collaborative Cross - the most genetically diverse mouse population in the world, which is generated, housed, and distributed from UNC. Traditional lab mice are much more limited in their genetic diversity, and so they have limited use in studies that try to home in on important aspects of diseases in humans. The Collaborative Cross bred together various wild type mice to create wide diversity in the mouse genome. Pardo-Manuel de Villena says this diversity is comparable to the variation found in the human genome. This helps scientists study diseases that involve various levels of genetic expression across many different genes.
Gene expression connects DNA to proteins, which then carry out various functions inside cells. This process is crucial for proper human health. Mutations that alter gene expression are called regulatory mutations.
"This type of genetic variation is probably the most important contributor - not to simple Mendelian diseases where there's just one gene mutation [such as cystic fibrosis] - but to much more common and complex diseases, such as diabetes, heart disease, neurological conditions, and a host of others," Pardo-Manuel de Villena says. "These diseases are driven by gene expression, not of one gene but of hundreds or thousands of genes.
"The Collaborative Cross and the expertise we have at UNC allow us to look at different gene expression for every gene in the genome of every kind of tissue," says Pardo-Manuel de Villena, who directs the Collaborative Cross.
For every gene a scientist is interested in, Pardo-Manuel de Villena's team can create mice that have low, intermediate, or high expression of genes. And they can explore if that expression is associated with a specific disease.
"This expression level is dependent on the mother or the father," Pardo-Manuel de Villena says. "We now know that mammals express more genetic variance from the father. So imagine that a certain kind of mutation is bad. If inherited from the mother, the gene wouldn't be expressed as much as it would be if it were inherited from the father. So, the same bad mutation would have different consequences in disease if it were inherited from the mother or from the father."
These types of genetic mutations across hundreds of genes are hard to study and a major bottleneck to realizing the promises of the post-genome era. But Pardo-Manuel de Villena says, "Thanks to the Collaborative Cross, the mouse can be used to model how these genes work and how they impact health and disease in any kind of tissue in the body."
[Source: UNC Healthcare and UNC School of Medicine]