MAY 17, 2021 7:00 AM PDT

Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells

SPONSORED BY: Promega Corporation

Inflammation, a process that was meant to defend our body from infection, has been found to contribute to a wide range of diseases, such as chronic inflammation, neurodegenerative disorders—and more recently, COVID-19. The development of new tools and methods to measure inflammation is crucial to help researchers understand these diseases.

Cytokines—small signaling molecules that regulate inflammation and immunity—have recently become the focus of inflammation research due to their role in causing severe COVID-19 symptoms. In these severe cases, the patient’s immune system responds to the infection with uncontrolled cytokine release and immune cell activation, called the “cytokine storm”. Although the cytokine storm can be treated using established drugs, more research is needed to understand what causes this severe immune response and why only some patients develop it. 

Researchers can study the cytokine storm and other immune responses using cell-based models and assays that detect cytokine release. Currently, the traditional enzyme-linked immunosorbent assay (ELISA) is the most common assay for detecting cytokines. It uses labeled antibodies and a detection reagent that changes color based on the amount of cytokine present. 

While ELISAs are simple to implement, they can take up to 24 hours to generate results. They also include numerous manual wash and pipetting steps, which means you must use a lot of plates, pipet tips and other lab consumables. You’ll also end up spending a lot of time in the lab to detect your cytokine of interest.

Promega has developed a new immunoassay platform for quantifying targets, such as a cytokines, without the need for any wash steps. With the new Lumit™ Immunoassays, all you need to do is add labeled antibodies, wait one hour, then add the detection substrate and read the assay plate on a standard luminometer.

Lumit is based on the award-winning NanoBiT® complementation system. NanoBiT consists of two subunits—small bit (SmBiT) and large bit (LgBiT). These subunits have low affinity for each other and only form an active luciferase enzyme when they come into close proximity. To detect cytokines, primary antibodies to the target are labeled with SmBiT and LgBiT. In the presence of the target cytokine, SmBiT and LgBiT come together to form an active luciferase enzyme, which produces light proportional to the amount of cytokine present. 

The Lumit platform allows for faster and easier detection of cytokines, supporting researchers interested in understanding the cytokine storm, looking for modulators of the inflammasome, and other inflammation and immunity research. 

To learn more about performance of the Lumit™ IL-1β Assay and other cytokine immunoassays, download this poster: Rapid and Sensitive Determination of Cytokine Release from Cells without Sample Transfer 

About the Sponsor
  • With a portfolio of more than 4,000 products covering the fields of genomics, protein analysis and expression, cellular analysis, drug discovery and genetic identity, Promega is a global leader in providing innovative solutions and technical support to life scientists in academic, industrial and government settings.
You May Also Like
SEP 02, 2021
Immunology
Hobit Activates Cancer-Killing Immune Cells
SEP 02, 2021
Hobit Activates Cancer-Killing Immune Cells
Innate lymphoid cells, or ILCs, are specialized immune cells that are increasingly entering the research spotlight. Thes ...
SEP 08, 2021
Health & Medicine
Gene Linked to Endometriosis Could Lead to Non-Hormonal Treatment Options
SEP 08, 2021
Gene Linked to Endometriosis Could Lead to Non-Hormonal Treatment Options
Researchers from the University of Oxford found an association between neuropeptide S receptor 1 (NPSR1) gene variants a ...
SEP 23, 2021
Immunology
Enhanced Hamster Cells as Super Drug Factories
SEP 23, 2021
Enhanced Hamster Cells as Super Drug Factories
Antibodies are highly specialized proteins produced by the immune system that stick on to foreign invaders in the body w ...
OCT 26, 2021
Immunology
Two Different Arms, Twice the Cancer-Killing Potential?
OCT 26, 2021
Two Different Arms, Twice the Cancer-Killing Potential?
Our immune system has developed an arsenal of sophisticated molecular weapons to defend us against the continuous barrag ...
NOV 02, 2021
Immunology
Green Tea Isn't an Antioxidant After All (But It's Still Good for You)
NOV 02, 2021
Green Tea Isn't an Antioxidant After All (But It's Still Good for You)
Green tea has long been touted as the elixir of youth—high concentrations of chemicals called polyphenols were bel ...
NOV 11, 2021
Immunology
Malaria Researchers Make a Surprising Antibody Find
NOV 11, 2021
Malaria Researchers Make a Surprising Antibody Find
Researchers looking into the immunology of malaria infections have made an unexpected find that could ultimately lead to ...
Loading Comments...