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Trending / Microbiology / Rapid, Real-Time Detection of Contamination in Cell Cultures
AUG 15, 2023 2:30 PM PDT

Rapid, Real-Time Detection of Contamination in Cell Cultures

SPONSORED BY: Sartorius

Abstract

In this study, we used Sartorius Real-time PCR kits for the rapid detection of bacteria, fungi, and mycoplasma to establish that these assays can detect microbial contaminants, even in the presence of high cell backgrounds, ranging from 10 to 40 million cells per milliliter (cells/mL). We observed that the cell density limit and assay robustness depend on cell types and media compositions. The detection limits for bacteria, fungi, and mycoplasma in Jurkat cells, HPBMC and CHO cells varies between 10 and 25 x 106 cells/mL.

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Sartorius
The Sartorius Group is a leading international partner of biopharmaceutical research and the industry. With innovative laboratory instruments and consumables, the Group's Lab Products & Services Division concentrates on serving the needs of laboratories performing research and quality control at pharma and biopharma companies and those of academic research...
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