MAY 09, 2017 7:00 AM PDT

WEBINAR: Advanced assays for high-throughput assessment of ion channel inhibition and cardiac toxicity

Sponsored by: Molecular Devices
Speakers

Abstract

DATE: May 9th, 2017
TIME: 7:00AM PDT, 10:00AM ET, 2:00PM GMT

 

Early detection and elimination of new chemical entities with cardiac or hERG liability could substantially improve drug safety and minimize the cost of development. In this webinar, we will highlight different assays for validating ion channel inhibition using cell lines and cardiac toxicity using induced pluripotent stem cells (iPSCs).

High-throughput screening for in vitro potency evaluation of cardiac ion channel inhibitors on the FLIPR Tetra® System

Learning Objectives:

  • Optimize and validate a robust cell-based hERG fluorescence assay using the FLIPR® Potassium Assay Kit, a thallium-sensitive fluorescence dye to directly measure ion influx
  • Run inhibition assays with voltage-gated sodium channels (NaV1.5) and L-type voltage-gated calcium channels (CaV1.2) using FLIPR® Membrane Potential Dye and FLIPR® Calcium 6 Dye, respectively
  • Demonstrate high-throughput, early stage in vitro compound and potency assessment with comparable results from electrophysiological recording data

 

Multi-parameter in vitro assessment of compound effects on cardiomyocyte physiology using induced pluripotent stem cells

Learning Objectives:

  • Determine the effects of pharmacological compounds and other chemicals on the beating rate and profiles of cardiomyocytes using the FLIPR Tetra system and ImageXpress® Micro system. Assay data is characterized from 2D and 3D spheroid cardiomyocyte cultures. The assays employ calcium sensitive dyes to monitor changes in intracellular Ca2+ fluxes synchronous with cell beating which allows monitoring of beat rate, amplitude, and other parameters.
  • Identify methods for multiparametric analysis and ranking of compounds for potential cardiotoxic hazards. This methodology is well suited for testing environmental chemicals and early safety testing in drug development prior to clinical studies.

 


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
NOV 17, 2021 8:00 AM PST
C.E. CREDITS
NOV 17, 2021 8:00 AM PST
Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
SEP 29, 2021 11:00 AM PDT
C.E. CREDITS
SEP 29, 2021 11:00 AM PDT
Date: Septembr 29, 2021 Time: 11:00am (PDT), 2:00pm (EDT) 3D cell models are becoming increasingly popular for studying complex biological effects, tissue functionality, and diseases. While...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
MAY 09, 2017 7:00 AM PDT

WEBINAR: Advanced assays for high-throughput assessment of ion channel inhibition and cardiac toxicity

Sponsored by: Molecular Devices


Show Resources
Loading Comments...
Show Resources