MAY 09, 2017 7:00 AM PDT

WEBINAR: Advanced assays for high-throughput assessment of ion channel inhibition and cardiac toxicity

Sponsored by: Molecular Devices
Speakers
  • Field Applications Scientist, Molecular Devices
    Biography
      Beiyan Zou is a Field Applications Scientist at Molecular Devices supporting reagents, FlexStation3, FLIPR and automated electrophysiology platforms. She received a Ph.D. degree in Biology at Pennsylvania State University and completed postdoctoral training in the Department of Neuroscience at John Hopkins University School of Medicine. She has 18 years of experience in molecular biology/genetics, 8 years of experience in ion channel biology, 4 years of experience in cellular imaging, and 8 years of experience in assay development and high throughput screen including 5 years of experience as a project leader at the Johns Hopkins Ion Channel Center, one of the nine NIH roadmap program funded screening centers. She has authored and coauthored in 20 peer-review papers in prestigious journals including PNAS, Cell, Journal of Neuroscience, Molecular Pharmacology and Assay Drug Development Technologies.
    • Senior Research Scientist, Molecular Devices
      Biography
        Oksana Sirenko earned her PhD in Biochemistry from the Biochemistry Research Institute, Academy of Sciences in Ukraine. For over 15 years, she has worked as a Research Scientist in cell-based assay development, drug discovery, and drug development in several pharma and biotech companies including Novartis, Bayer, and Fibrogen. At Molecular Devices she is focused on assay development using high-content imaging systems and other instruments to develop methods for cell-based disease modeling and toxicity research.

      Abstract

      DATE: May 9th, 2017
      TIME: 7:00AM PDT, 10:00AM ET, 2:00PM GMT

       

      Early detection and elimination of new chemical entities with cardiac or hERG liability could substantially improve drug safety and minimize the cost of development. In this webinar, we will highlight different assays for validating ion channel inhibition using cell lines and cardiac toxicity using induced pluripotent stem cells (iPSCs).

      High-throughput screening for in vitro potency evaluation of cardiac ion channel inhibitors on the FLIPR Tetra® System

      Learning Objectives:

      • Optimize and validate a robust cell-based hERG fluorescence assay using the FLIPR® Potassium Assay Kit, a thallium-sensitive fluorescence dye to directly measure ion influx
      • Run inhibition assays with voltage-gated sodium channels (NaV1.5) and L-type voltage-gated calcium channels (CaV1.2) using FLIPR® Membrane Potential Dye and FLIPR® Calcium 6 Dye, respectively
      • Demonstrate high-throughput, early stage in vitro compound and potency assessment with comparable results from electrophysiological recording data

       

      Multi-parameter in vitro assessment of compound effects on cardiomyocyte physiology using induced pluripotent stem cells

      Learning Objectives:

      • Determine the effects of pharmacological compounds and other chemicals on the beating rate and profiles of cardiomyocytes using the FLIPR Tetra system and ImageXpress® Micro system. Assay data is characterized from 2D and 3D spheroid cardiomyocyte cultures. The assays employ calcium sensitive dyes to monitor changes in intracellular Ca2+ fluxes synchronous with cell beating which allows monitoring of beat rate, amplitude, and other parameters.
      • Identify methods for multiparametric analysis and ranking of compounds for potential cardiotoxic hazards. This methodology is well suited for testing environmental chemicals and early safety testing in drug development prior to clinical studies.

       


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