DEC 17, 2020 9:00 AM PST

Advancing Cell Biology with Cryo-Correlative Microscopy

Sponsored by: Leica Microsystems, Alveole
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Professor of Biochemistry; Affiliate, Morgridge Institute for Research University of Wisconsin-Madison (USA)
    Biography

      Liz received her Ph.D. in Chemistry from Emory University. She engineered elastin-mimetic materials that are used for drug delivery and tissue engineering applications. She was a postdoctoral research associate in materials science at the University of Southern California. She was a postdoctoral scholar with Professor Grant Jensen at Caltech where she developed cryo-ET technologies and used cryo-ET to study HIV-1 maturation. She joined Emory University as an Assistant Professor in 2008 and was promoted to Associate Professor in 2016. She moved to the University of Wisconsin, Madison as a full Professor in 2018. Her research program focuses on the development and use of cryo-EM and correlative light and electron microscopy (CLEM) imaging technologies to determine the native-state structures of several bacterial species, bacteriophages, HIV-1, respiratory syncytial virus (RSV), measles virus (MeV), and other host-pathogen systems.

    • Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)
      Biography

        Jae completed her Ph.D. under the mentorship of Dr. Esther Bullitt at Boston University. She studied the ultrastructure and complexity of infectious extracellular vesicles released from cells infected by non-enveloped positive-sense RNA viruses using cryo-ET and mass spectrometry. After graduation, Jae joined Dr. Wright's group at UW-Madison and has focused on development of high-precision cryo-CLEM with flexible workflows and an easy-to-use software toolkit. She also works on cryo-EM sample optimization through micro-patterning and improvement of freezing conditions for cell-virus and cell-bacteria interactions, VLPs for vaccine development, and purified viral glycoproteins.

      • Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)
        Biography

          Bryan completed his Ph.D. under the mentorship of Dr. Paul Ahlquist at UW-Madison. He studied the organization and function of viral proteins essential for assembly of positive-strand RNA virus membrane-associated RNA replication complexes. Bryan is a postdoctoral research associate in Dr. Wright's lab at the UW-Madison. His research interests center around using cryo-ET to study viral structure and assembly in multiple systems including RSV, HIV-1, and Ty1 LTR-retrotransposon.

        • Graduate Student, Wright Laboratory University of Wisconsin-Madison (USA)
          Biography
            B.S. in Biochemistry and Biophysics, Oregon State University (USA)

            Joseph completed his undergraduate studies at Oregon State University in Biochemistry & Biophysics, conducting molecular biology research under Dr. Hong Moulton. He is currently a 2nd year physical chemistry Ph.D. student in Dr. Wright's lab at UW-Madison. His research interests involve using CLEM and cryo-ET to answer cell function and dysfunction questions in neuroscience. He uses both neuron-like cell lines and primary neuron cultures in his research.

          Abstract
          Date:  December 17, 2020
          Time: 9:00am (PST),  12:00pm (EST)
           
          Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with cryo-electron tomography allows investigators to bridge knowledge gaps between cell and structural biology. CLEM technologies are rapidly advancing in order to improve biological understanding, experimental reproducibility, and automation. Throughout the webinar, we will highlight insights that have been gleaned in several biological systems studied using CLEM workflows. We will review some of the basics of the technologies and provide examples of optimal workflows. We will also discuss some of the procedures for cell care, patterning substrates, culturing cells on substrates, and cryo-CLEM imaging.
           
          Learning Objectives
          • Discussion of results from infectious disease and neuroscience research.
          • Review of the equipment and techniques necessary for CLEM success in cell biology.
          • Summarize how to define and optimize important parameters to improve results, including Tips & Tricks.
           
           
          Webinars will be available for unlimited on-demand viewing after live event.
           
          LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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          Advancing Cell Biology with Cryo-Correlative Microscopy

          Sponsored by: Leica Microsystems, Alveole
          C.E. Credits: P.A.C.E. CE Florida CE

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