Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with cryo-electron tomography allows investigators to bridge knowledge gaps between cell and structural biology. CLEM technologies are rapidly advancing in order to improve biological understanding, experimental reproducibility, and automation. Throughout the webinar, we will highlight insights that have been gleaned in several biological systems studied using CLEM workflows. We will review some of the basics of the technologies and provide examples of optimal workflows. We will also discuss some of the procedures for cell care, patterning substrates, culturing cells on substrates, and cryo-CLEM imaging.

 

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