DEC 17, 2020 9:00 AM PST

Advancing Cell Biology with Cryo-Correlative Microscopy

Sponsored by: Leica Microsystems, Alveole
C.E. Credits: P.A.C.E. CE Florida CE
  • Professor of Biochemistry; Affiliate, Morgridge Institute for Research University of Wisconsin-Madison (USA)

      Liz received her Ph.D. in Chemistry from Emory University. She engineered elastin-mimetic materials that are used for drug delivery and tissue engineering applications. She was a postdoctoral research associate in materials science at the University of Southern California. She was a postdoctoral scholar with Professor Grant Jensen at Caltech where she developed cryo-ET technologies and used cryo-ET to study HIV-1 maturation. She joined Emory University as an Assistant Professor in 2008 and was promoted to Associate Professor in 2016. She moved to the University of Wisconsin, Madison as a full Professor in 2018. Her research program focuses on the development and use of cryo-EM and correlative light and electron microscopy (CLEM) imaging technologies to determine the native-state structures of several bacterial species, bacteriophages, HIV-1, respiratory syncytial virus (RSV), measles virus (MeV), and other host-pathogen systems.

    • Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)

        Jae completed her Ph.D. under the mentorship of Dr. Esther Bullitt at Boston University. She studied the ultrastructure and complexity of infectious extracellular vesicles released from cells infected by non-enveloped positive-sense RNA viruses using cryo-ET and mass spectrometry. After graduation, Jae joined Dr. Wright's group at UW-Madison and has focused on development of high-precision cryo-CLEM with flexible workflows and an easy-to-use software toolkit. She also works on cryo-EM sample optimization through micro-patterning and improvement of freezing conditions for cell-virus and cell-bacteria interactions, VLPs for vaccine development, and purified viral glycoproteins.

      • Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)

          Bryan completed his Ph.D. under the mentorship of Dr. Paul Ahlquist at UW-Madison. He studied the organization and function of viral proteins essential for assembly of positive-strand RNA virus membrane-associated RNA replication complexes. Bryan is a postdoctoral research associate in Dr. Wright's lab at the UW-Madison. His research interests center around using cryo-ET to study viral structure and assembly in multiple systems including RSV, HIV-1, and Ty1 LTR-retrotransposon.

        • Graduate Student, Wright Laboratory University of Wisconsin-Madison (USA)
            B.S. in Biochemistry and Biophysics, Oregon State University (USA)

            Joseph completed his undergraduate studies at Oregon State University in Biochemistry & Biophysics, conducting molecular biology research under Dr. Hong Moulton. He is currently a 2nd year physical chemistry Ph.D. student in Dr. Wright's lab at UW-Madison. His research interests involve using CLEM and cryo-ET to answer cell function and dysfunction questions in neuroscience. He uses both neuron-like cell lines and primary neuron cultures in his research.

          Date:  December 17, 2020
          Time: 9:00am (PST),  12:00pm (EST)
          Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with cryo-electron tomography allows investigators to bridge knowledge gaps between cell and structural biology. CLEM technologies are rapidly advancing in order to improve biological understanding, experimental reproducibility, and automation. Throughout the webinar, we will highlight insights that have been gleaned in several biological systems studied using CLEM workflows. We will review some of the basics of the technologies and provide examples of optimal workflows. We will also discuss some of the procedures for cell care, patterning substrates, culturing cells on substrates, and cryo-CLEM imaging.
          Learning Objectives
          • Discussion of results from infectious disease and neuroscience research.
          • Review of the equipment and techniques necessary for CLEM success in cell biology.
          • Summarize how to define and optimize important parameters to improve results, including Tips & Tricks.
          Webinars will be available for unlimited on-demand viewing after live event.
          LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

          Show Resources
          You May Also Like
          DEC 02, 2020 8:00 AM PST
          C.E. CREDITS
          DEC 02, 2020 8:00 AM PST
          DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
          OCT 08, 2020 7:00 AM PDT
          C.E. CREDITS
          OCT 08, 2020 7:00 AM PDT
          DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
          SEP 10, 2020 9:00 AM PDT
          C.E. CREDITS
          SEP 10, 2020 9:00 AM PDT
          Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
          DEC 16, 2020 8:00 AM PST
          C.E. CREDITS
          DEC 16, 2020 8:00 AM PST
          Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
          NOV 16, 2020 8:00 AM PST
          C.E. CREDITS
          NOV 16, 2020 8:00 AM PST
          Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
          NOV 18, 2020 8:00 AM PST
          C.E. CREDITS
          NOV 18, 2020 8:00 AM PST
          DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
          DEC 17, 2020 9:00 AM PST

          Advancing Cell Biology with Cryo-Correlative Microscopy

          Sponsored by: Leica Microsystems, Alveole
          C.E. Credits: P.A.C.E. CE Florida CE




          Research And Development

          Molecular Biology

          Cell Biology



          Molecular Genetics




          Cancer Research


          Laboratory Testing



          North America53%



          South America2%



          Registration Source

          Website Visitors52%

          Email Promotions14%

          Client/Sponsor Promotions12%

          LabRoots Registration (Also interested in)11%

          WAVE Newsletter Promotion7%

          Trending Newsletter Promotion2%

          Website Banners1%

          Job Title


          Medical Laboratory Technician19%

          Research Scientist13%




          Post Doc5%

          Clinical Laboratory Scientist4%

          Medical Doctor/Specialist4%

          Facility/Department Manager4%


          Genetic Counselor2%

          Lab Management2%



          Academic Institution39%

          Clinical Laboratory15%


          Research Institute5%


          Biotech Company3%

          Life Science Company2%

          Diagnostic Company2%

          Medical Center2%

          General Laboratory2%


          Medical School1%

          Independent Laboratories1%

          Non-Profit Organization1%


          Show Resources
          Loading Comments...
          Show Resources
          • See more