Anatomy of a nucleic acid sample preparation

There are many methods of nucleic acid isolation. Each technology offers different approaches of purification of the template. However, it is important to control carry over of “process contaminants”. This can manifest with altered sample quantitation and/or unusual downstream reaction results. Being mindful of the upstream contributing factors helps with understanding the template for downstream applications inherent to each unique technology.

Learning Objectives:

1. Molecular biotechnology techniques: nucleic acid sample preparation and quantitation