SEP 11, 2018 10:00 AM EDT

Answering the Challenges of Cell Viability and Reporter Gene Assays Screening Cost and Quality

SPONSORED BY: PerkinElmer
C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Global Product Leader and European Scientific Application Leader at PerkinElmer
    Biography
      Vincent Dupriez is Global Product Leader and European Scientific Application Leader at PerkinElmer, based in Brussels, Belgium.

      Vincent Dupriez holds an engineering degree (1991) and a Ph.D. in agronomic sciences and biological engineering (1997) from the University of Louvain, Belgium. His Ph.D. training was done at the "Christian de Duve Institute of Cellular Pathology" (ICP) and University of Louvain Medical School. He joined Euroscreen (Belgium) in 1998, where his group was in charge of Molecular Biology and Assay Development. In particular, he developed the AequoScreen methodology for High Throughput Screening in internal and collaborative drug discovery programs. He also was project manager for a CCR5 anti-HIV drug discovery program. He joined PerkinElmer in July 2007, following its acquisition of Euroscreen, and worked as an R&D member until December 2011, being involved in internal research and collaborations with external partners like Axxam, LONZA, Corning and TGR Biosciences. In January 2012, he transitioned to new functions in Business Development and Scientific Support for Europe. Since November 2013, Vincent is product manager for the "lites" luminescence reporter gene and cell viability assays, for the "SureFire" cellular phosphoproteins assays, and in Europe for the "Onpoint" custom assay developments and custom labeling businesses. Since 2005, Vincent is also lecturer at the free university of Brussels, for the PHARMED post-graduate lectures in pharmaceutical medicine/drug development sciences and since 2016 for the Clinix post-graduate lectures.
    • Senior Application Scientist at PerkinElmer
      Biography
        Jeanine Hinterneder is a Senior Application Scientist at PerkinElmer, based in Hopkinton, Massachusetts. Jeanine received her PhD from Brandeis University in Neuroscience in 2009 where her thesis work examined the mechanisms by which neurotrophic factors influence neuronal development. She joined PerkinElmer in 2010 as a member of the Technical Support team responsible for supporting life science research product lines, including microplates, high throughput screening (HTS) and radiometric reagents, and microfluidic instruments. Jeanine transitioned into the Discovery Applications group in 2013 and, in this role, has worked on developing new applications and protocols for the microfluidic and HTS product lines, authoring numerous Application Notes, Technical Notes and User Guides. She has presented her research at several professional conferences, including Discovery on Target, the Society for Lab Automation and Screening (SLAS) and the American Academy for Cancer Research (AACR). The goal of her research is to develop assays for novel targets and target classes utilizing in vitro assays with increased biological relevance, including, more recently, developing applications in 3D cell culture models grown in ultra-low attachment spheroid microplates.
      • Research Associate, Fraunhofer IME Screening Port, Hamburg
        Biography
          Maria Kuzikov studied Molecular Life Science at the University of Lübeck. She worked on the development of inhibitors targeting virus replication at the Department of Biochemistry of University of Lübeck and on assay development for studying protein-protein interactions at the European ScreeningPort. After obtaining her Master of Science degree in 2013, Maria continued to work at European ScreeningPort (now Fraunhofer IME ScreeningPort) and was responsible for development of cell-based and biochemical assays in High Throughput Screening (HTS) formats, as well as High Content Assays and early ADME-Tox profile studies. This work includes the analysis of ion channels, P450 enzymes and G-protein coupled receptors (GPCRs) as well as cell lines and primary cells throughout all stages of HTS using numerous assay formats like luminescence, IF, label-free, proximity-ligation or TR-FRET.

        Abstract:

        DATE: Tuesday, September 11, 2018

        TIME: 10:00AM EDT, 16:00 CET

        With increasing safety requirements, and time and financial pressure, the development of new drugs, and the execution of basic research projects, are presenting multiple challenges. To be successful in such a competitive environment, it is more important than ever to select the right approach to execute the different aspects of the multiple tasks needed to develop a new drug or to execute fundamental research. In this Webinar, examples will be presented where different technologies have been compared to analyze the potency of transcription inhibitors, and to measure the cytotoxic potential of test compounds in relevant cellular models, using various assay and reader platforms. The relative advantages of each method will be discussed, and the key points important for the project efficiency and cost will be highlighted. Dr. Vincent Dupriez (PerkinElmer) will first make a general introduction, and will then present data generated by collaborators, about “Improving dual reporter gene assays quality and efficiency: use of twinlite for studying transcriptional regulation by viral inhibitor proteins.”  And “Selecting the best option for PBMC luminescent cell viability assays.” Dr. Maria Kuzikov (Fraunhofer IME ScreeningPort) will present the data generated at the Fraunhofer IME ScreeningPort about “An efficient method, space and cost-saving, to assess cell viability using the ATPlite reagent and the VICTOR Nivo™ Multimode Plate Reader and will discuss the advantages of using this platform. Dr. Jeanine Hinterneder (PerkinElmer) will speak about “Improving the predictability of cytotoxicity tests by moving to 3D cell culture models.” And will illustrate the different 3D cell culture options to grow spheroids and the methods developed to analyze the viability of such spheroids.

        Learning Objectives:

        • Learn about differences in reporter gene assays options, and their impact on the cost, quality and ease of execution of R&D work
        • Learn about different options for cell viability testing, the impact of such differences on the execution of R&D work, and about the possibility to run such assays on relevant cellular models.

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