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Nov 12, 2020

Fully Automated IHC Multiplexing: Techniques and Assay Development

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • DISCOVERY Reagent & Training Lead, Companion Diagnostics (CDx) & DISCOVERY, Roche Tissue Diagnostics
    Biography

      Mr. Hellewell has been with Roche Tissue Diagnostics (RTD) for 8 years where he has held several positions within the company. He began his career with RTD UK where he held sales and applications positions. In 2016 he relocated to Tucson, AZ to take up a global role with customer support for RTD. In 2017 he joined the DISCOVERY International Business team as Global Reagent and Training Lead, where he manages their reagent portfolio and global DISCOVERY training activities for customers and RTD personnel.


    Abstract

    Multiplexing IHC is a technique that is increasing in demand as more and more researchers and physicians are being tasked with doing more with less. With multiplexing, you have the advantage to view different targets and their levels of expression in relation to one another. In this presentation, we will review the different technologies and techniques that are currently available to assist with developing a multiplex assay. After a brief overview of the technologies, we will apply some of those technologies and walk through an example of optimizing a multiplex assay. Although digital analysis of multiplexed slides can be an important factor in the interpretation of a multiplexed assay, this presentation will focus on and emphasize the staining aspects of the assay optimization.

    Learning Objectives: 

    1. Understand the utility and methods for multiplexing

    2. Understand the principles behind multiplex assay development


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