AUG 29, 2017 9:00 AM PDT

Automated Optimization of IgG Production in CHO Cells

Speakers

Abstract

DATE: August 29th, 2017

TIME:  9:00am PT, 12:00pm ET

 

Antibodies are frequently produced from a cell line that has been screened to ensure the protein is expressed with the desired post-translational modifications, target specificity and affinity, and at relatively high levels. The identified cell line can be further developed to achieve higher titers and to increase cell growth and/or protein production through the optimization of the cell culture media. An ideal approach to media optimization is through the use of a factorial design of experiment (DOE), where a variety of media components are tested at different concentrations and in combination with one another. The challenge though, is that these factorial experiments rapidly increase the number of conditions that require testing. It is desirable therefore to use instrumentation that can minimize the time required for sample preparation and quantification during the optimization process.

Common ways of quantifying the production of IgG or other proteins are either labor-intensive (e.g. ELISAs) or prohibitively slow (e.g. HPLC), particularly at the throughput required for DOE studies. A rapid alternative for quantification and kinetics analysis of biologics is the high-throughput Octet HTX platform that utilizes Bio-Layer Interferometry to detect real-time binding of molecules.  Here we show how coupling this high-throughput analysis with the Biomek FXP Automated Workstation enabled the DOE optimization of IgG expression in a CHO cell line. The Biomek FXP Workstation was used to prepare 96 media combinations and plate cells in replicate conditions, generate sample and reagent plates for the Octet HTX system, and initiate XTT assays to better understand the effect of media components on CHO cell growth.


You’ll learn:

  • How a statistical design of experiment approach can be automated to accelerate the optimization of media for protein production.
  • How the Octet HTX can rapidly measure IgG concentrations in a high-throughput manner for screening applications.
  • How integrating a liquid handler with additional instruments can enable the complete automation of complex workflows.

Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
JUL 13, 2021 11:00 AM PDT
C.E. CREDITS
JUL 13, 2021 11:00 AM PDT
Date: July 13, 2021 Time: 11:00am (PDT), 1:00pm (EDT) 1 R&D, Cell Culture & Fermentation Sciences, Bio Pharmaceutical Development, AstraZeneca, Gaithersburg, USA, 2 R&D, ADPE, Ce...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
AUG 29, 2017 9:00 AM PDT

Automated Optimization of IgG Production in CHO Cells


Specialty

Biotechnology

Molecular Biology

Immunology

Cell Biology

Antibodies

Cancer Research

Cell Culture

Biochemistry

Assay Development

Cancer Therapeutics

Biomarkers

Infectious Disease

Molecular Diagnostics

Genetics

Microbiology

Geography

North America52%

Europe22%

Asia17%

South America3%

Africa3%

Oceania1%

Registration Source

Website Visitors100%

Job Title

Research Scientist18%

Scientist16%

Facility/Department Manager14%

Student13%

Executive11%

Educator/Faculty6%

Post Doc5%

Medical Laboratory Technician4%

Lab Management4%

Clinical Laboratory Scientist2%

Medical Doctor/Specialist2%

QC/QA2%

Engineer2%

Marketing/Sales2%

Organization

Biotech Company11%

Academic Institution8%

Life Science Company5%

Research Institute3%

Hospital3%

Clinical Laboratory2%

Ambulatory Care2%

Pharmaceutical Company2%

Non-Profit Organization1%

Diagnostic Company1%

Contract Research Organization (Cro)1%

Consultant1%

Medical School1%

Manufacturer - Other1%

Other4%


Show Resources
Loading Comments...
Show Resources