AUG 29, 2017 09:00 AM PDT

Automated Optimization of IgG Production in CHO Cells

Speakers
  • Staff Applications Scientist, Beckman Coulter Life Sciences
    Biography
      Mike Kowalski is a Staff Applications Scientist in the Sample Preparation and Applied Markets group of Beckman Coulter Life Sciences. He received his Ph.D. in Microbiology and Molecular Genetics from Harvard University. Prior to joining Beckman, Mike was a postdoctoral fellow at the Novartis Institutes for Biomedical Research, where he used automation to screen for novel regulators of stem cell pluripotency and differentiation. Since joining Beckman, Mike has developed automated applications in the areas of cell culture, cellular analysis, and mass spectrometry.
    • Marketing Applications Manager - ForteBio, A Division of Pall Life Sciences
      Biography
        David Apiyo is a Marketing Applications Manager with Pall Fortebio in charge of Manufacturing, Bioprocess and QC applications. He previously worked as an Applications Scientist and a technical Specialist for Fortebio. David received his Ph.D. in Chemistry at Tulane University and held two postdoctoral stints at Rice University and at the Pacific NorthWest National Lab (PNNL). Prior to joining Pall Fortebio, he worked as a Senior Development Scientist at Beckman Coulter where he functioned as a technical lead in various projects within the Discovery and Technology Group.

      Abstract:

      DATE: August 29th, 2017

      TIME:  9:00am PT, 12:00pm ET

       

      Antibodies are frequently produced from a cell line that has been screened to ensure the protein is expressed with the desired post-translational modifications, target specificity and affinity, and at relatively high levels. The identified cell line can be further developed to achieve higher titers and to increase cell growth and/or protein production through the optimization of the cell culture media. An ideal approach to media optimization is through the use of a factorial design of experiment (DOE), where a variety of media components are tested at different concentrations and in combination with one another. The challenge though, is that these factorial experiments rapidly increase the number of conditions that require testing. It is desirable therefore to use instrumentation that can minimize the time required for sample preparation and quantification during the optimization process.

      Common ways of quantifying the production of IgG or other proteins are either labor-intensive (e.g. ELISAs) or prohibitively slow (e.g. HPLC), particularly at the throughput required for DOE studies. A rapid alternative for quantification and kinetics analysis of biologics is the high-throughput Octet HTX platform that utilizes Bio-Layer Interferometry to detect real-time binding of molecules.  Here we show how coupling this high-throughput analysis with the Biomek FXP Automated Workstation enabled the DOE optimization of IgG expression in a CHO cell line. The Biomek FXP Workstation was used to prepare 96 media combinations and plate cells in replicate conditions, generate sample and reagent plates for the Octet HTX system, and initiate XTT assays to better understand the effect of media components on CHO cell growth.


      You’ll learn:

      • How a statistical design of experiment approach can be automated to accelerate the optimization of media for protein production.
      • How the Octet HTX can rapidly measure IgG concentrations in a high-throughput manner for screening applications.
      • How integrating a liquid handler with additional instruments can enable the complete automation of complex workflows.

      Show Resources
      You May Also Like
      MAY 16, 2019 04:00 PM CEST
      C.E. CREDITS
      MAY 16, 2019 04:00 PM CEST
      DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
      JUN 05, 2019 05:00 PM CEST
      C.E. CREDITS
      JUN 05, 2019 05:00 PM CEST
      DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
      NOV 18, 2019 07:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      NOV 18, 2019 07:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
      SEP 05, 2019 04:00 PM CEST
      C.E. CREDITS
      SEP 05, 2019 04:00 PM CEST
      DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
      OCT 02, 2019 11:00 AM PDT
      OCT 02, 2019 11:00 AM PDT
      DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
      AUG 13, 2019 09:00 AM PDT
      C.E. CREDITS
      AUG 13, 2019 09:00 AM PDT
      DATE: August 13, 2019TIME: 9:00am PT, 12:00pm ET, 5:00pm BST Molecular complexes are major constituents of cells, hence unraveling their mechanisms is key to fuller comprehension of c...
      Loading Comments...
      Show Resources