DATE: October 10, 2017
TIME: 9:00AM PDT

Patient-derived xenografts (PDX) are increasingly being used in biomedical research for therapeutic analysis in mice. Unlike traditional cell line xenografts, which are established from suspensions of cultured cells, PDX are maintained entirely in vivo. While exclusive in vivo maintenance is the primary advantage of PDX as a model for cancer, the practice introduces unique challenges and potential pitfalls to working with PDX as a model for cancer. Perhaps the most significant challenge to working with PDX is the need to disaggregate solid tumors prior to their use in downstream applications such as flow cytometry, immunomagnetic enrichment, single-cell sequencing or the establishment of cohorts for therapeutic trials. This webinar will discuss our experience with automated single cell dissociation of primary tumors and patient-derived xenografts as well as compatibility with downstream applications.

Learning Objectives:

• Advantages and challenges associate with using PDX models for cancer research

• Solid tumor dissociation methods compatible for downstream single-cell applications, such as flow cytometry, immunomagnetic enrichment and single-cell sequencing