The Basics of Protein Analysis Topic 1: Fundamental of antibodies for applied research Topic 2: Practical considerations for quantitation in western blotting

 

 

Antibodies are vital tools in many of the laboratory techniques that are used to answer basic research questions. Due to their high specificity and selectivity, antibodies have always had the potential to be of great use as biochemical tools for a range of applications including selection, identification, purification and as therapeutics. Being some of the most critical reagents used in the research lab, qualified validated antibodies will highly enhance research outcome by enabling superior performance for researchers. Along with ELISA, western blotting is still one of the most widely performed protein detection/immuno-detection applications. However, experimental variations such as inconsistent sample and target concentrations, unequal sample loading and protein transfer, saturated immuno-probing, and poor detection often affect quantitative results. In this webinar, we will refresh the knowledge on the fundamentals of antibodies for applied research as well as highlight some practical considerations for quantitation in Western Blotting. We will define the properties of different antibodies and optimized western workflow to help you generate reliable immunoassay data and publish your findings with more confidence.

 

Learning Objectives