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Beyond Automated iPSC reprogramming: applications for a fully automated cell culture system for pluripotent stem cell research

Speaker
  • Chief Technology Scientist, Centre for Brain development and Repair, Instem, Bangalore
    Biography
      Dr. Paull is Vice President, Automation Systems and Stem Cell Biology at The NYSCF Research Institute. Daniel oversees the day-to-day operation of The NYSCF Global Stem Cell Array in both the production of induced pluripotent stem cells and development of novel tools using the robotic platform including such areas as gene editing and differentiation. He also oversees a number of collaborative projects aimed at developing research across a range of disease areas. He received his PhD from University College London, England and followed this with post-doctoral work at the New York Stem Cell Foundation where he helped develop novel approaches for the treatment of mitochondrial disease as well as furthered research into somatic cell nuclear transfer.

    Abstract

    The use of pluripotent stem cells is dramatically altering the R&D landscape, providing new insights into both the basic biology of disease progression and novel cell types for pharmaceutical drug screening.  Additionally, pluripotent stem cells are already in clinical use for a number of disease indications. However, one limitation of the work to date is that in-vitro studies have largely focused on highly penetrant point mutations known to directly lead to a disease phenotype. While useful, this approach may not always be applicable to idiopathic versions of the same disease. For the majority of diseases, underwritten by common causal variants, modeling using only a limited number of cell lines is insufficient. To overcome these issues, we have developed a fully automated platform which allows high throughput cell biology to be accomplished: from reprogramming of hundreds of cell line in parallel to high throughput differentiations, large scale experiments can now be performed to interrogate these diseases. Here we report on progress in adapting the NYSCF Global Stem Cell Array technology to encompass high throughput genome editing as well as large-scale differentiations and screens. We will present a number of case studies highlighting the continued development of this automated system for stem cell biology.


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