MAY 26, 2015 6:00 AM PDT

Cell-Imaging and Luminescence Based Cytotoxicity Analysis using the EnSight Multimode Plate Reader

Speakers
  • Application Specialist, PerkinElmer
    Biography
      Frauke Haenel studied Biology and achieved her PhD in 2011 at the University of Kassel, Germany. She joined PerkinElmer in May 2011 as part of the application support team based in Hamburg, Germany. The team provides support for the Multimode Detection Plate Readers to customers, sales and service. Within this team, Frauke mainly focuses on providing assistance on imaging and label-free applications, including trainings and application development.
    • Chief Scientific Officer, Fraunhofer IME, Hamburg, Germany
      Biography
        Dr. Philip Gribbon is the Chief Scientific Officer at the Fraunhofer IME, a public-private partnership based in Hamburg Germany, which works mainly with academic research institutes in the area of drug discovery. The ESP uses a range of high throughput screening (HTS) systems and assay methods and provides support for biomarker studies within clinical and pre-clinical research projects. Since the setup of the ESP, Dr. Gribbon has worked in close collaboration with leading life-science industry partners, such as PerkinElmer, to help validate novel technologies which can directly apply to projects. Previously, Dr. Gribbon has worked at Pfizer Global Research on hit discovery using HTS technologies and gained considerable experience in drug discovery across several target classes. Later, at GSK, he led a team of physicists with the remit of increasing the impact of biophysical methods on the pharmaceutical R&D process. Dr. Gribbon did his undergraduate and PhD studies at Imperial College, London and served as a post-doctoral researcher at the University of Manchester, working on how the biophysical properties of proteoglycans help define the mechanical properties of cartilage in health and disease.

      Abstract

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      Cytotoxicity remains one of the major causes of drug withdrawal and there is an urgent need for reliable and time-saving assay workflows. Cells exposed to toxic compounds show a variety of phenotypes including the loss of membrane integrity, cell shrinkage and nuclear fragmentation. These are ideal effects to be analyzed using cell imaging in addition to conventional cytotoxicity assays.


       In the first part of this webinar, we will give you an overview of the EnSight™ Multimode Plate Reader with Well-Imaging Technology and its capabilities to analyze multiple parameters of cytotoxicity on the same sample using the Kaleido™ Data Acquisition and Analysis Software. In the second part of the webinar, we will show a real life application of both anti-cancer drug discovery and in-vitro toxicity testing. You will see how to determine the compound effects on cellular viability and proliferation by comparing the use of brightfield imaging and ATP detection.


       During this webinar, you will:


      -        Learn about the EnSight system’s capabilities to detect cytotoxicity using multiple parameters


      -        Get insight into automated image analysis using the Kaleido software


      -        See how live cell imaging can provide additional information and save you time and money


      -        Have your questions answered live


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