OCT 11, 2017 10:30 AM PDT

Characterization and application of the triple immunodeficient R2G2 (Rag2/Il2rg double knockout) mouse model

Sponsored by: Envigo
Speaker
  • Chief Scientific Officer - Biologics, Envigo
    Biography
      Lee was recruited by Huntingdon Life Sciences at the end of 2004 to take a leading role in working with customers developing biopharmaceutical products.

      Lee brought to the role hands-on experience of biopharmaceutical drug development gained in companies such as; Cantab Pharmaceuticals, Xenova and CellFactors. During his time in the biotechnology industry, he was involved in developing therapies including: vaccines, recombinant proteins; monoclonal antibodies; immunomodulatory molecules; viral and non-viral gene delivery systems; and cell-based therapies.

      He has a well-rounded knowledge of the regulatory environment for biologics and particular expertise in the analysis of protein and viral products.

      At Envigo, Lee is Chief Scientific Officer - Biologics. He is also a serving committee member on the Monoclonal Antibody Expert Working Group of the National Centre for the 3Rs, The BIA Manufacturing Advisory Committee, the RSC / RPS Joint Pharmaceutical Analysis Group the ABPI Nonclinical and Biological Discovery Expert Network (NaBDEN) committee.

    Abstract

    The “R2G2” (B6;129-Rag2tm1FwaIL2rgtm1Rsky/DwlHsd) knockout mouse is the latest advancement to provide an alternative option in the highly immunodeficient mouse model category for oncology, immunology and other biomedical research communities. This model was generated by backcrossing an IL2rg (common gamma) knockout model to a RAG2 (recombinase activating gene) knockout model. The resulting mouse lacks various cytokines including IL-2, IL-4, IL-7, IL-9 and IL-15. In addition, this model lacks B cells, T cells, NK cells and has a deficiency in lymphocyte development. The R2G2 mouse model is not only ultra immunodeficient, but provides a model that is less leaky and more tolerant to gamma radiation than traditional SCID models. Herein we describe the development and characterization of the R2G2 mouse model, which includes breeding history, growth curve, complete blood count and serum chemistry, flow cytometry, tumor growth and radiosensitivity.


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    OCT 11, 2017 10:30 AM PDT

    Characterization and application of the triple immunodeficient R2G2 (Rag2/Il2rg double knockout) mouse model

    Sponsored by: Envigo

    Specialty

    Dna

    Gene Expression

    Virology

    Cell Culture

    Immunology

    Lab Animals

    Immuno-Oncology

    Earth Science

    Animal Sciences

    Genetics

    Drug Discovery

    University

    Gene Editing

    Cancer Diagnostics

    Mass Cytometry

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    Europe50%

    Asia25%

    Registration Source

    Website Visitors100%

    Job Title

    Student50%

    Medical Laboratory Technician50%

    Organization

    Research Institute25%

    Academic Institution25%

    Manufacturer - Other25%


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