OCT 11, 2017 10:30 AM PDT

Characterization and application of the triple immunodeficient R2G2 (Rag2/Il2rg double knockout) mouse model

Sponsored by: Envigo
Speaker

Abstract

The “R2G2” (B6;129-Rag2tm1FwaIL2rgtm1Rsky/DwlHsd) knockout mouse is the latest advancement to provide an alternative option in the highly immunodeficient mouse model category for oncology, immunology and other biomedical research communities. This model was generated by backcrossing an IL2rg (common gamma) knockout model to a RAG2 (recombinase activating gene) knockout model. The resulting mouse lacks various cytokines including IL-2, IL-4, IL-7, IL-9 and IL-15. In addition, this model lacks B cells, T cells, NK cells and has a deficiency in lymphocyte development. The R2G2 mouse model is not only ultra immunodeficient, but provides a model that is less leaky and more tolerant to gamma radiation than traditional SCID models. Herein we describe the development and characterization of the R2G2 mouse model, which includes breeding history, growth curve, complete blood count and serum chemistry, flow cytometry, tumor growth and radiosensitivity.


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OCT 11, 2017 10:30 AM PDT

Characterization and application of the triple immunodeficient R2G2 (Rag2/Il2rg double knockout) mouse model

Sponsored by: Envigo

Specialty

Dna

Gene Expression

Virology

Cell Culture

Immunology

Animal Sciences

Lab Animals

Immuno-Oncology

Earth Science

Genetics

Drug Discovery

University

Gene Editing

Cancer Diagnostics

Mass Cytometry

Geography

Europe50%

Asia25%

Registration Source

Website Visitors100%

Job Title

Student50%

Medical Laboratory Technician50%

Organization

Research Institute25%

Academic Institution25%

Manufacturer - Other25%


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