OCT 30, 2014 3:00 PM PDT

Clinical Shotgun Proteomics in Cancer Biomarker and Drug Target Research/Discovery

Speakers
  • Senior Scientist, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research (FNLCR)
    Biography
      Dr. Blonder is the Clinical Proteomics Lead at the Cancer Research Technology Program at Frederick National Laboratory for Cancer Research (FNLCR), operated by Leidos Biomedical Research, Inc. For the National Cancer Institute. In 1978, Dr. Blonder received his M.D. at the Rijeka University School of Medicine, Croatia. In 2000, through Associated Western Universities, Dr. Blonder was awarded a post-doc fellowship in proteomics at the Pacific Northwest National Laboratory (PNNL), Richland, WA (Advisor: Dr. Richard D. Smith). In 2002, Dr. Blonder joined the Laboratory of Proteomics & Analytical Technology, at the FNLCR (formerly NCI-Frederick) where he continues to develop and apply MS-based proteomics to cancer research. Since 2006, he has led the Clinical Proteomics Group, extending his research to in-depth profiling of membrane proteome. In parallel, he worked on the methodology for cancer biomarker and drug target discovery using clinical specimens. His group was the first to optimize the immunodepletion of abundant proteins from tissue homogenates. In 2013, Dr. Blonder joined the Antibody Characterization Laboratory to lead the cell surface project within the Ras Initiative at the FNLCR/NCI focused on discovery of cell surface targets, enriched or unique to cancers driven by mutated RAS that could be targeted by drugs, antibodies, antibody-drug conjugates, or nanoparticles. This effort resulted in the first cell surface proteome map of a model cell line expressing oncogenic KRasG12V. Dr. Blonder brings unique combination of his expertise in clinical proteomics, medicine and systems biology, focusing his research on the development of innovative approaches for cancer biomarker and drug target discovery. He is an editor of the BMC Cancer and a lecturer at the Foundation for Advanced Education in the Sciences at NIH. Dr. Blonder has authored over 60 scientific publications in areas of biological mass spectrometry, clinical proteomics and cancer research.

    Abstract:

    Mass spectrometry (MS)-based profiling of clinical specimens has been increasingly used in cancer research to characterize changes in protein expression between tumor and healthy tissue or between the blood of diseased and healthy individuals. These molecular profiles may lead to distinct insights that are not readily evident using in vitro cultured cells or animal models and may facilitate discovery of clinically applicable biomarkers and novel drug targets. However, the discovery of valid cancer biomarkers and drug targets using MS-based proteomics has proven difficult, primarily due to the analytical challenges exemplified in the wide dynamic range of human plasma protein levels (i.e., > 10 orders of magnitude) and the fact that the 10 most abundant proteins constitute ~ 90% of the total plasma protein mass. Analytical challenges related to MS-based profiling of clinical samples obtained from diverse patient populations are complex, different from those in basic cancer research characterized by controlled experimental conditions employing in vitro cultured cells or transgenic animals. Therefore, experimental design and sample preparation represent the most challenging steps within a clinical proteomic workflow aimed at the development of reproducible and high-throughput methods for cancer biomarker and drug targets discovery. This presentation focuses on our recent advances in experimental design and method development using high resolution/accuracy MS-based proteomics for molecular profiling of clinical specimens in the context of cancer biomarker and novel drug targets research/discovery.


    Show Resources
    You May Also Like
    SEP 05, 2019 4:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 4:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    AUG 27, 2019 9:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 9:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    FEB 26, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    NOV 07, 2019 10:00 AM PST
    C.E. CREDITS
    NOV 07, 2019 10:00 AM PST
    DATE: November 7, 2019TIME: 10:00am PST, 1:00pm EST Studying the pathogenesis of diabetes requires detailed analysis of the pancreatic islet microenvironment and its numerous c...
    Loading Comments...
    Show Resources