NOV 14, 2017 09:00 AM PST

WEBINAR: CRISPRa tools for transcriptional activation studies

Speakers
  • Senior Scientist at Dharmacon, a Horizon Discovery Group company
    Biography
      Žaklina Strezoska is a Senior Scientist at Dharmacon, a Horizon Discovery Group company. She joined Dharmacon in 2007 and currently leads projects in multiple research areas including RNA interference, CRISPR-Cas9 genome engineering, and CRISPRa transcriptional modulation with a focus on innovation and new product development. She has extensive experience in arrayed and pooled screening and hit identification and validation. Prior to joining Dharmacon, she worked at ALTANA Pharma where she gained experience in target identification, validation, and small molecule discovery. Žaklina received her B.S. and M.S. degrees in Molecular Biology from Belgrade University and her PhD. Degree in Molecular Genetics at the University of Illinois at Chicago. She completed a postdoctoral fellowship at Brown University.
    • R&D Manager, Functional Genomic Screening, Horizon Discovery
      Biography
        Ben completed his PhD at the University of Manchester and trained as a post-doctoral scientist at the University of Cambridge studying reverse chemical genetic screening in the unfolded protein response. His research identified and defined the mechanism of a new tool compound against a key proteostasis kinase. Ben joined Horizon in 2013 to expand and advance Horizon's functional genomic screening capability and to lead a major research alliance in synthetic lethal target discovery. Ben led the development of pooled CRISPR-Cas9 screening at Horizon which was launched as a service in 2015. The addition of CRISPRi and CRISPRa followed in 2017 and Ben now manages the teams applying these functional genomic screening platforms in target discovery, validation and other research programmes.

      Abstract:

      DATE: November 14, 2017
      TIME: 09:00am PST, 12:00pm EST

      The CRISPR-Cas9 system has been adapted to upregulate any gene in its endogenous context, enabling overexpression experiments and avoiding the use of exogenous expression plasmids. The simplicity of programming this CRISPR activation (CRISPRa) system with small RNA guides is transformative for performing systematic gain-of-function studies. This exciting complement to loss-of-function studies (such as with RNAi and CRISPR-Cas9) also provides new tools to identify gene functions that might otherwise go undetected using a downregulation or knockout mechanism alone.

      With CRISPRa, the guide RNAs form a complex with a nuclease-deactivated Cas9 (dCas9) which is in turn fused to transcriptional activators. This machinery then acts at the transcription start sites to upregulate expression of a target gene. In this webinar, we will describe different CRISPRa technologies and provide experimental considerations for using either synthetic guide RNA (crRNA:tracrRNA) or lentiviral sgRNA, in either arrayed or pooled screening formats. These platforms can be applied in tandem to enable both rapid follow-up of screen hits and development of complementary assays for functional gene analysis.

      In this webinar you will learn:

      • The mechanisms of CRISPR activation
      • How to set up CRISPRa experiments using predesigned guide RNA, including delivery optimization and guide RNA format selection recommendations
      • The relationship between baseline gene expression and activation levels
      • The basics of using pooled lentiviral CRISPRa screening for target ID and validation

      Show Resources
      You May Also Like
      SEP 05, 2019 04:00 PM CEST
      C.E. CREDITS
      SEP 05, 2019 04:00 PM CEST
      DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
      MAY 16, 2019 04:00 PM CEST
      C.E. CREDITS
      MAY 16, 2019 04:00 PM CEST
      DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
      JUN 05, 2019 05:00 PM CEST
      C.E. CREDITS
      JUN 05, 2019 05:00 PM CEST
      DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
      NOV 18, 2019 07:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      NOV 18, 2019 07:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
      AUG 27, 2019 09:00 AM PDT
      C.E. CREDITS
      AUG 27, 2019 09:00 AM PDT
      DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
      JUL 31, 2019 09:00 AM PDT
      C.E. CREDITS
      JUL 31, 2019 09:00 AM PDT
      DATE: July 31, 2019TIME: 9:00am PT, 12:00pm ET The choroid plexus, which makes up the blood-cerebrospinal fluid barrier in the central nervous system (CNS), lines the ventricle...
      Loading Comments...
      Show Resources