OCT 03, 2018 9:00 AM PDT

CRISPRseek and GUIDEseq for Design of Target-Specific Guide RNAs in CRISPR-Cas9 Genome-Editing Systems

Presented at: CRISPR 2018
Speaker
  • Professor and Director of Bioinformatics Core, University of Massachusetts Medical School
    Biography
      Lihua Julie Zhu obtained her Ph.D. in Nutritional Sciences from the University of Wisconsin-Madison in 1999, and her M.S. in Computer Science from DePaul University in Chicago in 2001. She joined the Bioinformatics Core of the Robert H. Lurie Comprehensive Cancer Center (RHLCCC) of Northwestern University in 2001, where she was the Director of Bioinformatics Consulting Core from 2003 to 2005 and the Director of Clinical Informatics Group from 2005 to 2007. She joined the University of Massachusetts Medical School (UMMS) as the head of Bioinformatics core of Program in Gene Function and Expression (PGFE) in 2007. Dr. Zhu is currently a professor and the head of Bioinformatics Core in the Department of Molecular, Cell and Cancer Biology (MCCB) of UMMS.

    Abstract

    The most recently developed genome editing system, CRISPR-Cas9 has greater inherent flexibility than prior programmable nuclease platforms. Because of its simplicity and efficacy, this technology is revolutionizing biological studies and holds tremendous promise for therapeutic applications. However, imperfect cleavage specificity of CRISPR-Cas9 nuclease within the genome is a cause for concern for its therapeutic application. To facilitate the adoption and improvement of this technology, we have developed CRISPRseek for designing target specific gRNAs, and GUIDEseq for identifying genome-wide offtarget sites from GUIDE-seq and CIRCLE-seq experiments to assess the precision of engineered CIRSPR-Cas9 nucleases. In this talk, I will give an introduction to the CRISPR genome editing, GUIDE-seq and CIRCLE-seq technologies, followed by an overview of the functionalities of CRISPRseek and GUIDEseq. By the end of talk, the participants should be able to design target-specific gRNAs for various cas9 nucleases and genomes using CRISPRseek, and analyze GUIDE-seq and CIRCLE-seq data using GUIDEseq.

    Learning Objectives: 

    1. Understand how the CRISPR-Cas9 Genome-Editing system is recolutionizing biological studies
    2. Understand how to design target-specific gRNAs 


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