MENU
APR 29, 2021 9:00 AM PDT

Cryo-Electron Microscopy: membrane proteins down to atoms

Speakers

Abstract

Date:  April 29, 2021

Time: 9:00am PDT

 

Cryo-electron microscopy (cryo-EM) reached single-atom resolution for 3D structure determination of biological macromolecules. Cryo-EM has been determining the structures of protein structures for soluble as well as membrane proteins that have a key role in human disease. It has become possible to obtain sufficiently high resolution structures to visualize water molecules, hydrogen atoms and in the cases of optimized samples, individual protein atoms. For protein structures, especially membrane proteins, improvements in resolution below 3 Å would mean improved rotamer assignments due to better defined side chain densities, visualization of bound drug or ligand molecules and an unambiguous assignment of structurally ordered water molecules that are very important for any structure-based drug discovery. The resolution leap is possible due to cryo-EM technological advances. We will review how our 300 kV, cryo-transmission electron microscope (Thermo Fisher Krios G4) works from the cold field emission source to detector and Selectris Imaging Filter. We will also show brand new results on membrane protein structures going beyond 2.5 Å resolution obtained with our 200kV Glacios cryo-TEM.

 

Learning Objectives

  • Discover the implications of atomic resolution cryo-EM for structural biology
  • Explore recent research on membrane structures from both 200kV and 300kV cryo-TEM
  • Tour a cryo-EM microscope from cold field emission source, detectors and image/energy filtering

 

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


Show Resources
You May Also Like
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
SEP 29, 2022 8:00 AM PDT
SEP 29, 2022 8:00 AM PDT
Date: September 29, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Adeno-associated virus (AAV) is one of the most widely used delivery vehicles in gene therapy. To ensure the safety...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
Loading Comments...
Show Resources