FEB 05, 2014 04:00 PM PST

Cryopreservation: a cold solution

Speakers
  • Independent Consultant
    Biography

       
      Jorge Sztein is a veterinarian of the Universidad Nacional de La Plata (Argentina) and Doctor in Sciences of the same university specializing in laboratory animals (1991). Then after working at the National Academy of medicine(1987-1989, Buenos Aires), start in 1990 as a post doctoral student at the NIH (National Institutes of Health of the United States) creating transgenic animals and performing cryopreservation techniques. In 1995 the Jackson Labs hires me as head of research in Cryobiology and also has duties as professor in the cryo-courses of the institute in USA, Italy, France and Spain. In the year 2000 I returned to the NEI-NIH as Colonies Manager and head of cryopreservation and then in the 2008 move to another Institute (NIAID) as director of cryopreservation and assisted reproduction till July2013. Today I'm an independent consultant on cryopreservation and Assisted reproduction.
       

    Abstract:

    The popularity reached by the genetic manipulation of laboratory animals to create new models for studying human diseases, produced in turn, that the techniques for assisted reproduction consolidate as routine in the laboratory of an animal facility. However, the costs of maintaining these mouse strains have increased deeply, and the physical space to housed these animals is practically the same one, for what we should admit that it is a concrete problem. In order to alleviate this problem is why embryo cryopreservation took importance in the early 90s. The cryopreservation technology has the advantage of being a tool to control the animal space at a low cost, but also has the benefits of being able to stop the risk of possible natural mutations, and genetic or infectious contamination (a tragic epidemic).Nowadays we can freeze embryos, sperm and ovaries to cover any situation of protecting the genetics of a mouse line. At the same time, the progress in the manipulation of the sperm and in the methods of in vitro fertilization does this task a more efficient process. With the sperm collected from a mouse there is enough material to fertilize approximately thousand of oocytes, which facilitates the task to preserve, to rederivate, and to expand or to distribute a mouse strain. Another application of this technology is to recover strains that otherwise it could be lost or by negligence, lack of breeding or by death. Today it is also possible to recover sperm from a death mouse and fertilize in-vitro up to 4 days after his dead.


    Show Resources
    You May Also Like
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    MAY 02, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 02, 2018 08:00 AM PDT
    Immunohistochemistry protocols, which utilize antibodies to visualize proteins in tissue sections, have many steps that need optimized to prevent non-specific background effects, artifacts, o...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    AUG 15, 2018 08:00 AM PDT
    C.E. CREDITS
    AUG 15, 2018 08:00 AM PDT
    DATE: August 15, 2018TIME: 08:00AM PDT, 11:00AM EDTThe failure of current chemotherapeutic strategies in the fight against cancer can be largely attributed to the occurrence of drug res...
    Loading Comments...