Development of Capture Sequencing Assays for Enhanced Detection and Genotyping of Tick-borne Pathogens and Infectious Vertebrate Viruses

C.E. Credits: Florida CE P.A.C.E. CE
Speaker

Abstract

Early detection is critical for prompt and effective treatment of acute infectious diseases. For tick-borne diseases (TBD), the lack of accurate early diagnosis can result in delayed treatment, augment morbidity, and increase the likelihood of developing persistent symptoms. For diagnosis of Lyme disease, the low sensitivity of molecular assays in the majority of clinical presentations has prevented their extensive implementation as an effective diagnostic tool. Serology remains the primary means of diagnosis for Lyme disease and the majority of TBD. TBDCapSeq is a sequencing assay that uses hybridization capture probes that cover the complete genomes of the eleven most common tick-borne agents found in the United States. We evaluated the performance of TBDCapSeq to surveil samples that included human whole blood, mouse tissues, and field-collected ticks. For Borrelia burgdorferi and Babesia microti, the sensitivity of TBDCapSeq was comparable and occasionally exceeded the performance of agent-specific quantitative Polymerase Chain Reaction (PCR) and resulted in 25 to > 10,000-fold increase in pathogen reads when compared to standard unbiased sequencing.
 
Current virus diagnostic assays that are commonly based on PCR assays targeting one or a few specific agents are limited in scope, may fail to detect virus variants and provide only limited genotypic information. Our assay VirCapSeq-VERT addresses many of the current challenges of PCR and sequencing for diagnostics and virome analysis. The sensitivity and specificity of the VirCapSeq-VERT are comparable to those of agent-specific real-time PCR. 
 

 

Learning Objectives:

1. Limitations of microbial diagnostic molecular assays for genomic and pathogenesis studies of tick-borne and viral agents.

2. Offer valuable new insights into our understanding of tick-borne and viral diseases by facilitating analysis of previously challenging specimens using standard sequencing.


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
C.E. CREDITS
The Human Microbiome Project was conceived almost 15 years ago, as an extension of the Human Genome Project, to explore the diversity of human-associated microorganisms at multiple body site...
JUN 08, 2021 9:00 AM PDT
JUN 08, 2021 9:00 AM PDT
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
Loading Comments...
Show Resources