OCT 11, 2016 7:00 AM PDT

Two Dimensional Liquid Chromatography Applied to the Characterization of Monoclonal Antibodies and Antibody-Drug Conjugates

Speaker

Abstract
DATE:  October 11, 2016
TIME:  7:00am PT, 10:00am ET

Monoclonal antibodies (mAbs) have emerged as important therapeutics for the treatment of life-threatening diseases. The successes of mAbs have triggered the development of various next generation formats. In oncology, antibody-drug conjugates (ADCs) are particularly promising, since they synergistically combine a specific mAb linked to a biologically active cytotoxic drug via a stable linker. The promise of ADCs is that highly toxic drugs can selectively be delivered to tumor cells thereby substantially lowering side effects as typically experienced with classical chemotherapy.

Monoclonal antibodies have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This challenge becomes even bigger in case ADCs are considered since the heterogeneity of the initial antibody is superimposed with the variability associated with the cytotoxic drug conjugation strategy. The present webinar will highlight the power of two dimensional liquid chromatography in hyphenation to high resolution mass spectrometry for the characterization of monoclonal antibodies and antibody-drug conjugates. Both multiple heart-cutting LC-LC, applied at protein level, and comprehensive LCxLC, applied at peptide level, will be discussed.

For Research Use Only. Not for use in diagnostic procedures.

Learning Objectives:
  • Obtain insight in the complexity associated with monoclonal antibodies and antibody-drug conjugates.
  • Learn about the challenges and strategies associated with the characterization of monoclonal antibodies and antibody-drug conjugates.
  • Learn about the different modes of two dimensional liquid chromatography and its hyphenation to mass spectrometry.

Show Resources
You May Also Like
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
SEP 13, 2022 8:00 AM PDT
SEP 13, 2022 8:00 AM PDT
Date: September 13, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Preparedness against pandemic diseases demands rapid-response vaccine technology and ready-to-use analytical methods...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
JUN 01, 2022 9:00 AM PDT
JUN 01, 2022 9:00 AM PDT
Date: June 01, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) The manufacturing process for cell and gene therapies is complex and can contain process-related impurities from multiple...
JUN 22, 2022 10:00 AM PDT
JUN 22, 2022 10:00 AM PDT
Date: June 22, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) Rabbits have a robust and unique immune system that enables the generated antibodies with superior specificity, affinity,...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
Loading Comments...
Show Resources