OCT 11, 2016 07:00 AM PDT

Two Dimensional Liquid Chromatography Applied to the Characterization of Monoclonal Antibodies and Antibody-Drug Conjugates

Speakers
  • Scientific Director, Research Institute for Chromatography (RIC), Kortrijk, Belgium
    Biography
      Koen Sandra received a PhD degree in Biochemistry from the Ghent University, Belgium in 2005. After his PhD, he joined Pronota, a molecular diagnostics company where he was active in developing analytical platforms for disease biomarker discovery and in setting up external collaborations. In 2008, he joined the Research Institute for Chromatography (RIC), a company that provides chromatographic and mass spectrometric support to the chemical, life sciences and pharmaceutical industries, where he currently holds the position of Scientific Director. He is furthermore co-founder and co-owner of anaRIC, a company that offers a complete range of analytical solutions for characterization, quality control, release and stability testing of biological drugs and of Metablys, an institute performing metabolomics and lipidomics research. As a non-academic scientist, Koen Sandra is author of over 40 highly cited scientific papers and has presented his work at numerous conferences as an invited speaker

    Abstract:
    DATE:  October 11, 2016
    TIME:  7:00am PT, 10:00am ET

    Monoclonal antibodies (mAbs) have emerged as important therapeutics for the treatment of life-threatening diseases. The successes of mAbs have triggered the development of various next generation formats. In oncology, antibody-drug conjugates (ADCs) are particularly promising, since they synergistically combine a specific mAb linked to a biologically active cytotoxic drug via a stable linker. The promise of ADCs is that highly toxic drugs can selectively be delivered to tumor cells thereby substantially lowering side effects as typically experienced with classical chemotherapy.

    Monoclonal antibodies have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This challenge becomes even bigger in case ADCs are considered since the heterogeneity of the initial antibody is superimposed with the variability associated with the cytotoxic drug conjugation strategy. The present webinar will highlight the power of two dimensional liquid chromatography in hyphenation to high resolution mass spectrometry for the characterization of monoclonal antibodies and antibody-drug conjugates. Both multiple heart-cutting LC-LC, applied at protein level, and comprehensive LCxLC, applied at peptide level, will be discussed.

    For Research Use Only. Not for use in diagnostic procedures.

    Learning Objectives:
    • Obtain insight in the complexity associated with monoclonal antibodies and antibody-drug conjugates.
    • Learn about the challenges and strategies associated with the characterization of monoclonal antibodies and antibody-drug conjugates.
    • Learn about the different modes of two dimensional liquid chromatography and its hyphenation to mass spectrometry.

    Show Resources
    You May Also Like
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    AUG 15, 2018 08:00 AM PDT
    C.E. CREDITS
    AUG 15, 2018 08:00 AM PDT
    DATE: August 15, 2018TIME: 08:00AM PDT, 11:00AM EDTThe failure of current chemotherapeutic strategies in the fight against cancer can be largely attributed to the occurrence of drug res...
    SEP 12, 2017 08:00 AM PDT
    SEP 12, 2017 08:00 AM PDT
    DATE: September 12, 2017TIME: 8:00am PT, 11:00am ETEvery year, millions of dollars are wasted on poorly characterized and performing antibodies.  Key researchers in the antibody co...
    Loading Comments...