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Directed differentiation of hematopoietic cells from human pluripotent stem cells

Speaker
  • Research Associate, Center of Regenerative Medicine in Barcelona
    Biography
      Dr. Giorgetti holds a Bachelor of Biology (1998) and PhD in Molecular Medicine from the University of Milan, Italy. She has a long-standing interest in hematopoietic stem cells, which started in her PhD thesis. She continued her studies in Dr. Rafii´s Lab at the Weill Cornell Medical College in New York. In 2008 she joined Center of Regenerative Medicine of Barcelona (CMRB). Her work contributed to the molecular understanding of nuclear reprogramming (Cell Stem Cell 2009; Nature Protocols 2010; Nature 2011, Stem Cells 2011, PNAS 2012, Nature Commun 2013). In 2012, she joined Inbiomed as Head of the Laboratory of Hematopoiesis and Blood Disorders. In 2013, she relocated to the Josep Carreras Leukaemia Research Institute where she contributed to develop new strategies for the in vitro generation of blood cells (Stem Cell Reports 2016; Exp Hematol 2017; Stem Cells 2017). Recently she relocated to the CMR[B] where she has just established herself as Team Leader.

    Abstract

    The continuous generation of blood cells throughout life relies on the existence of hematopoietic stem cells (HSC) generated during embryogenesis. Given the importance of HSC transplantation in cell-based therapeutic approaches, considerable efforts have been made toward understanding the developmental origins of embryonic HSC. Adult-type HSC are first generated in the aorta-gonad-mesonephros (AGM) region between days 27 and 40 of human embryonic development. It is relatively well accepted that the HSC emerge in the AGM through a hemogenic endothelium, but the direct precursor of this cell type remains to be clearly identified. The current picture of human hematopoietic development is largely extrapolated from animal models (mouse and zebrafish), as comparable studies on the human embryo are not possible. Human pluripotent stem cells (hPSCs) offer unprecedented opportunity to complement this knowledge, assuming that differentiation in the culture dish can recapitulate the dynamic and complexity of hematopoietic development in vivo. Here we summarize the recent advances made to understand the origins of hematopoietic stem cells in the early embryo and we discuss in detail latest protocols to obtain HSC from hPSCs.


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