JUL 28, 2021 8:00 AM PDT
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Disease model generation: 5 steps to a 3D cancer spheroid model

Speaker

Abstract

Date:  July 28, 2021

Time: 8:00am PDT

 

This presentation highlights a 5-step outline for the development of a 3D spheroid model using breast cancer as an example. These guidelines can be extrapolated to cells of other cancer types as well.

Since R&D initiatives provide a lot of novel concepts to drug development and growth of the cancer therapeutics market, it is important to have relevant model systems to assess drug response. As such, three-dimensional (3D) cell cultures of cancer have increasingly become the model of choice for basic as well as preclinical research in cancer biology.  

The generation of organoids and spheroids for cancer, includes:

  • Choosing the appropriate cell and tissue types
  • Optimizing growth and maintenance conditions for the 3D cultures
  • Characterizing the models
  • Assessing drug responses on such models to better understand the physiological effects of such drugs

This presentation will highlight a 5-step outline for the development of a 3D spheroid model using breast cancer as an example, however these guidelines can be extrapolated to cells of other cancer types as well.

 

Learning Objectives

  • The workflow to generate 3D cancer cell models
  • Learn the different assays available to characterize your developing cancer 3D cell model
  • Explore imaging and analysis tools to assess drug responses

 

 

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