Distinct Metabolic Profiling of Invading PANC-1 Pancreatic Cancer Cells

C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Senior Investigator, National Institutes for Quantum and Radiological Science and Technology, Japan
    Biography
      Dr. Mayumi Fujita received her PhD from the University of Tokyo, Japan, and has been working at National Institutes for Quantum and Radiological Science and Technology in Japan since 2008. She also joined the lab of Dr. David A. Wink at the National Institute of Health last year and investigated the role of nitric oxide in invading cells. She studies the mechanism of cancer cell invasion and developed a real-time imaging model of invading cells.

    Abstract:

    During this webcast Dr. Mayumi Fujita of the National Institutes for Quantum and Radiological Science and Technology in Japan will address the method of real-time imaging of invading cells using the IncuCyte Live-Cell Analysis System, and describe how this model is useful for many studies.

    In previous studies, only 1% of cells in the cultured human pancreatic cancer cell line, PANC-1, were capable of invasion through MatrigelÆ in a transwell invasion assay. This suggested that invasive PANC-1 cells have unique characteristics conferring this phenotype. This was further investigated using a 3D spheroid model of PANC-1, embedded in Matrigel, coupled with IncuCyteÆ Live Cell imaging and analysis to capture the movement of the distinct invading population in real time. The identified, invasive PANC-1 were collected and metabolically characterized by CE-TOFMS, and their metabolic profile compared with whole-culture PANC-1. (Fujita et al. 2017, Cancer Science). The invasive PANC-1 cells were distinct from those of the whole cultured PANC-1, with demonstrated increased consumption of ATP, assumed activation of ATP-generating pathways, and higher arginine utilization by NOS. Although they had higher oxidative stress, the invading cells were also more resistant to it with greater survival upon exposure to H2O2. A reduction of intracellular GSH by BSO inhibited PANC-1 invasiveness. Taken together, these results provide a unique metabolic profile for this invasive PANC-1 cell population, as compared to control cells. Such methodology is readily available for similar assessments of invasive cell phenotypes.

    You will learn:

    • A method will be provided for the real-time imaging of cancer cell invasion from 3D spheroid using the IncuCyteÆ Live-Cell Analysis System.
    • The utility of this model to study cancer cell invasion will be discussed, describing methods of invasion, including that by single cells, collected cells, elongated invasion, or rounded invasion.
    • Important methods information for real-time image capture, identification of invasive phenotypes, and integration with metabolomics analysis.
    • How to easily apply the use of IncuCyte technology and associated applications to their cell line of interest for related studies.

    Show Resources
    You May Also Like
    MAR 05, 2019 09:00 AM PST
    C.E. CREDITS
    MAR 05, 2019 09:00 AM PST
    DATE:  March 5, 2019TIME:  09:00am PST, 12:00pm EST MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post tran...
    FEB 26, 2019 09:00 AM PST
    C.E. CREDITS
    FEB 26, 2019 09:00 AM PST
    DATE:  February 26, 2019TIME:   9:00am PST, 12:00pm EST In an era of increasingly high-throughput, large-scale biology, with companies, government and non-prof...
    MAY 23, 2019 09:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 23, 2019 09:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE:  May 23, 2019TIME:   9:00am PDT, 12:00pm EDT Although mesenchymal stem/stromal cells (MSCs) chondrogenic differentiation has been thoroughly investigated...
    MAR 26, 2019 02:00 PM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAR 26, 2019 02:00 PM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE:  March 26, 2019TIME:  2:00pm PDT, 5:00pm EDT  Prostate cancer is the second most common form of cancer in males, affecting one in eight men by the time t...
    FEB 20, 2019 09:00 AM PST
    C.E. CREDITS
    FEB 20, 2019 09:00 AM PST
    DATE:  February 20, 2019TIME:  9:00am PST  ...
    APR 23, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    APR 23, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: April 23, 2019TIME: 11:00am PDT, 2:00pm PDT Cataract, a clouding of the ocular lens, is the leading cause of blindness worldwide. Currently the only means of treatment i...
    Loading Comments...
    Show Resources