MAY 12, 2016 10:30 AM PDT

DNA methylation analysis by multiplexed reduced representation bisulfite sequencing

Speaker

Abstract

DNA methylation is an essential mechanism of epigenetic gene regulation with broad relevance in development and disease. Its localization on genomic DNA and general stability make this epigenetic mark an attractive target for large-scale studies in cancer research, developmental biology and ecology. In recent years, microarray-based technologies have been gradually replaced by more robust, accurate and versatile next generation sequencing-based methods. 
    
This presentation will introduce highly-multiplexed reduced representation bisulfite sequencing as a cost-effective means for large DNA methylation studies. The protocol provides a single-base resolution read-out of 5-methylcytosine, while avoiding the cost of whole-genome sequencing. Based on an enzymatic enrichment step, the method is cost-effective, but provides excellent coverage of promoter regions, CpG islands, and other genomic elements such as enhancers and CpG island shores. At the same time, the protocol supports high-throughput applications, is suitable for any vertebrate species and has been optimized specifically for formalin-fixed, paraffin-embedded samples.

In our lab, we have successfully used RRBS on over 2000 samples comprising many different vertebrate species, various cancers, FFPE and low-input samples, and are happy to share our experience with the epigenomics community.

Learning objectives:

  • the talk will introduce highly multiplexed reduced representation bisulfite sequencing as a cost-effective means for large DNA methylation studies
  • get an overview of the required bioinformatic workflow, for an easy transition from microarrays to next generation sequencing-based analyses

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MAY 12, 2016 10:30 AM PDT

DNA methylation analysis by multiplexed reduced representation bisulfite sequencing


Specialty

Pcr/rt-Pcr/real-Time Pcr

Molecular Biology

Dna Sequencing

Molecular Diagnostics

Laboratory Testing

Dna

Biochemistry

Clinical Diagnostics

Genomics

Cell Biology

Gene Sequencing

Cancer

Systems Biology

Immunology

Cell Culture

Geography

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Europe25%

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Website Visitors100%

Job Title

Clinical Laboratory Scientist75%

Student25%

Organization

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Academic Institution25%

Medical School25%


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