Droplet Digital PCR as a tool for gene therapy application

Presented at: BioProcessing 2020
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Scientist, Technical Operations, Sana Biotechnology
    Biography
      Nagarjun is a Scientist in Technical Operations at Sana Biotechnology (Cambridge, MA). He completed his PhD in Medical Sciences at Texas A&M University (Texas), where he worked on development of a universal lentiviral vector platform for different gene therapy applications. After his graduate school, he joined Lonza (Houston), as a research intern and worked on developing cell lines for high titer productivity of different viral vectors. Nagarjun currently works on analytical method development to develop fit for purpose genomic assays to characterize viral vectors across Sana gene therapy platforms.

    Abstract

    Digital PCR has become the emerging technique for the sequence-specific detection and quantification of nucleic acids for various applications. During the recent years, numerous articles of new digital PCR methods have been published. Considering the development work into reliable analytical methods suitable for diagnostics requires their validation for the intended use. The results of an in-house evaluation of a droplet digital PCR compared to other technologies will be presented in application to different genomic assays applied in gene therapy.

    Learning Objectives:

    1) Introduction to Droplet Digital PCR

    2) Explain the need for Droplet Digital PCR compared to current methodologies in different gene therapy platforms

    3) Present the key evaluations required to establish the assay for research and good manufacturing purpose.


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