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NOV 16, 2016 4:00 PM PST

Evolving your media system for modern stem cell applications

Speaker
  • Senior Staff Scientist, Cell Biology, Thermo Fisher Scientific
    Biography
      Dr. Newman joined the Primary and Stem Cell Systems Division Life Technologies in 2009 and initially focused on isolation and expansion of human primary cells for development of 3D organotypic models. Currently, Dr. Newman continues to support primary cell efforts and works on development of next generation stem cell culture and differentiation systems, enabling researchers to efficiently culture, expand, cryopreserve, and differentiate their stem cells to various cellular lineages. She received a Ph.D. at the University of Iowa from the lab of Dr. Madeline Shea, focusing on ligand induced allostery of calmodulin and its impact on regulation of the Ryanodine Receptor Type 1. Subsequently, she completed postdoctoral training in the lab of Dr. Ken Prehoda at the University of Oregon, studying the role of intramolecular interactions in regulating cell signaling cascades in the process of assymetric stem cell division.

    Abstract

    Culture systems for pluripotent stem cell (PSC) expansion enable generation of a nearly unlimited pool of cells for downstream differentiation, disease modeling, drug discovery, and therapeutic applications.  While a number of PSC feeder-free medium systems exist, there are many challenges encountered by stem cell scientists across the PSC workflow in today’s applications.  Here we sought to improve the robustness and versatility of traditional PSC culture medium systems identify and optimize critical medium components.  Through assessment of over 65 different formulations, an optimum medium composition was identified which provides compatibility across the PSC workflow from somatic cell reprogramming, PSC expansion, downstream differentiation, as well as providing support in stressful applications such as gene editing.  This system additionally provides versatility, allowing for every-other-day or weekend-free feed schedules and compatibility with a broad range of passaging reagents and matrices.  Together this system provides a robust next-generation stem cell medium system for today’s challenging PSC workflow needs.  For Research Use Only


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