DEC 10, 2019 08:00 AM PST
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Expi293: New Tools for Structural Biology and Difficult to Express Proteins

Speakers
  • Director, Cell Biology, Life Sciences Solutions Group, Thermo Fisher Scientific
    Biography
      Jonathan Zmuda is a Director of Cell Biology at Thermo Fisher Scientific in Frederick, Maryland. Jon leads a team dedicated to discovering and developing new technologies and products useful for cell biology applications, including transient protein expression, advanced cell culture, and rare-cell analysis. He received his Ph.D. in Cell Biology from the University of Maryland, College Park.

    Abstract:
    DATE:  December 10, 2019
    TIME:  8:00am PST, 11:00am EST
     
    In this presentation, we demonstrate the performance of a new range of Expi293 components, including engineered Expi293 cell lines (e.g. GNTI-, Inducible and Inducible/GNTI- Expi293 cell lines) to allow for regulated expression and/or glycosylation of proteins, as well as an Expi293 Methionine-deficient system for metabolic labeling of proteins for NMR or multiwavelength anomalous dispersion (MAD). Together, these new tools significantly enhance the ability of researchers to produce proteins for structural biology analyses.
     
    Learning Objectives:
    • Explain why the new Expi293 tools for glycan modulation, inducible expression, and protein labeling are suitable for structural biology and challenging protein application areas.
    • Discuss how to optimize expression of challenging proteins, including GPCRs and ion channels
     
     
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