Expi293: New Tools for Structural Biology and Difficult to Express Proteins

Sponsored by: Thermo Fisher Scientific/Gibco

Speaker

Jon Zmuda, PhD

Director, Cell Biology, Life Sciences Solutions Group, Thermo Fisher Scientific
BIOGRAPHY

Event Date & Time

DATE: December 10, 2019

TIME: 8:00am PST, 11:00am EST

Abstract

In this presentation, we demonstrate the performance of a new range of Expi293 components, including engineered Expi293 cell lines (e.g. GNTI-, Inducible and Inducible/GNTI- Expi293 cell lines) to allow for regulated expression and/or glycosylation of proteins, as well as an Expi293 Methionine-deficient system for metabolic labeling of proteins for NMR or multiwavelength anomalous dispersion (MAD). Together, these new tools significantly enhance the ability of researchers to produce proteins for structural biology analyses.

Learning Objectives:

Explain why the new Expi293 tools for glycan modulation, inducible expression, and protein labeling are suitable for structural biology and challenging protein application areas.
Discuss how to optimize expression of challenging proteins, including GPCRs and ion channels

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