MENU
MAY 16, 2018 8:00 PM HKT

ExpiCHO High-Efficiency Protein Expression System: Ultra-high Titers Comparable to Stable Transfection Systems Achieved in Two Weeks (Presented in Chinese)

Speaker

Abstract

ExpiCHO is a highly efficient transient expression system that provides an expression level comparable to stable cell lines within two weeks. While CHO cells have been widely used in the industry for a long time, stable cell lines must be constructed to obtain CHO-expressed proteins, a time-consuming and labor-intensive procedure that requires experienced R&D personnel. In the drug development phase, it is necessary to produce a large quantity of proteins in a relatively short period of time, which makes the 293-transient expression system the only choice in most cases. This system, however, expresses at low levels with inconsistencies in the quality of the yielded proteins compared with the CHO system. In contrast, ExpiCHO, as an efficient transient expression system, can reach a yield of at least 1 g/L in just two weeks. In 2017, we used this system for the production of multiple fusion proteins and monoclonal antibodies. The expression levels were maintained at 1 g/L or more, exceeding 2 g/L and up to 3 g/L in some cases. When comparing the expression levels of the transient expression platform with those of stable CHO-S cell lines, we found that the two systems were  similar. In addition, while expressing a large amount of a protein, the transient system could also evaluate the expression level of the molecule in stable cell lines. ExpiCHO therefore provides an excellent tool for users who need to prepare a high volume of proteins rapidly.

ExpiCHO是一种高效的瞬时表达系统,可以在2周内获得与稳定细胞株相当的表达量。 CHO细胞长期以来一直在工业界广泛使用,获得CHO表达蛋白必须构建稳定的细胞株,这一过程不仅耗时耗力,而且需要经验丰富的研发人员。在药物开发阶段需要在较短时间获得大量蛋白,往往只能采用293细胞的瞬转表达系统,但该系统产量低,表达蛋白与CHO系统存在质量差异。ExpiCHO作为高效的瞬时表达系统能够在2周时间内获得克级每升表达量。2017年,我们采用该系统进行了多种融合蛋白及单抗的生产,表达量均维持在1g/L以上,部分超过2g/L,接近3g/L水平。同时,我们对比了瞬转表达水平与基于CHO-S的稳定细胞株表达水平,发现瞬转表达系统的产量与稳转细胞株的产量类似,瞬转系统在制备大量蛋白的同时也能评估该分子在稳定细胞株的表达水平。因此,ExpiCHO为需要快速制备大量蛋白的用户提供了一种极好的工具。


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
FEB 24, 2021 10:00 AM PST
C.E. CREDITS
FEB 24, 2021 10:00 AM PST
DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
APR 01, 2021 8:00 AM PDT
C.E. CREDITS
APR 01, 2021 8:00 AM PDT
Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
JUN 08, 2021 9:00 AM PDT
JUN 08, 2021 9:00 AM PDT
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
MAY 16, 2018 8:00 PM HKT

ExpiCHO High-Efficiency Protein Expression System: Ultra-high Titers Comparable to Stable Transfection Systems Achieved in Two Weeks (Presented in Chinese)


Specialty

Biotechnology

Antibodies

Cell Culture

Cell Biology

Molecular Biology

Cancer Research

Biochemistry

Immunology

Cancer Diagnostics

Gene Expression

Bioinformatics

Crispr-Cas9

Biomedical Engineering

Cancer Therapeutics

Biomarkers

Geography

Asia65%

North America24%

Europe5%

South America4%

Oceania1%

Registration Source

Website Visitors89%

WAVE Newsletter Promotion7%

Email Promotions1%

Website Banners1%

Client/Sponsor Promotions1%

Social Media Promotions1%

Job Title

Research Scientist20%

Student20%

Executive11%

Educator/Faculty8%

Scientist8%

Facility/Department Manager6%

Medical Laboratory Technician6%

Lab Management5%

Engineer4%

Post Doc3%

Medical Doctor/Specialist3%

Clinical Laboratory Scientist2%

Biologist1%

Veterinarian1%

Organization

Biotech Company26%

Academic Institution16%

Medical School11%

Research Institute7%

Clinical Laboratory5%

Pharmaceutical Company5%

Life Science Company4%

Government3%

Medical Center3%

Non-Profit Organization2%

Industrial Company1%

Ambulatory Care1%

Contract Research Organization (Cro)1%

Hospital1%

Other5%


Show Resources
Loading Comments...
Show Resources