SEP 02, 2015 3:00 PM PDT

Fundamentals and Clinical Applications of Biofilms and Rapid Diagnostic Technique

Presented at: Microbiology
Speaker
  • Medical Director, Southwest Regional Wound Care Center
    Biography
      Dr. Randall Wolcott is the founder of the Southwest Regional Wound Care Center in Lubbock, TX. The Wound Care Center specializes in biofilm-based wound care. The Wound Care Center offers state of the art technology with molecular diagnostics and state of the art alternative medicine including customized topical wound gels. He is passionate about healing wounds.

    Abstract

    Biofilm is an ancient and preferred mode of growth for microbes with 99% of all microbes residing in a biofilm. Biofilm is characterized by a community of microbes (usually polymicrobial often termed diversity) which constructs a structure composed of polymers (sugars, proteins and/or DNA) to form a protective matrix around the community. This matrix allows the individual microbes within the biofilm to differentiate for different functions such as the environmental edge (which senses and reacts at a molecular level to the environment), a region with highly efficient and upregulated horizontal gene transfer between microbes (stress response) and a deep region which accumulates metabolic waste products and is devoid of oxygen which has been used up by the microbes of the biofilm. The basal region with an anoxic core causes microbes to become dormant and selects for persister cells while also providing an excellent environment for strict anaerobic bacteria to propagate. Biofilm phenotype mode of growth, along with the protective matrix, confers many defenses for the biofilm including high resistance to antibiotics and biocides, very limited penetration of host immune mechanisms such as white blood cells, antibodies and compliment and the ability to regrow the entire biofilm after a catastrophic event such as debridement. It is the characteristics of biofilm which presents clinically as a chronic infection and the chronic infection of skin is the chronic wound. Most wound care is done by trial and error because of lack of diagnostic capabilities. Current clinical cultures are designed to find one organism growing in planktonic phenotype. Biofilm, on the other hand, is polymicrobial (diversity) whose microbial constituents are viable (still alive) yet not culturable. Therefore, clinical culture methodologies are wholly unsuited for diagnosing wound biofilm. Therefore, molecular methods such as PCR and sequencing have been utilized to identify and quantitate the microorganisms present in biofilm. By utilizing real-time PCR the approximate number of microbes per gram of tissue can be determined (bacterial load). Sequencing is necessary to identify fungus and bacteria that are present in the wound and their relative contribution (percent of each species) to the biofilm. This allows the determination of dominant species and the most important targets for topical and/or systemic antibiotics and biocides. By appropriately identifying the microorganisms present in the biofilm, and then suppressing those microorganisms, wound healing outcomes are improved.


    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    MAR 16, 2021 10:00 AM PDT
    C.E. CREDITS
    MAR 16, 2021 10:00 AM PDT
    Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    APR 01, 2021 8:00 AM PDT
    C.E. CREDITS
    APR 01, 2021 8:00 AM PDT
    Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
    SEP 02, 2015 3:00 PM PDT

    Fundamentals and Clinical Applications of Biofilms and Rapid Diagnostic Technique

    Presented at: Microbiology

    Specialty

    Animal Behavior

    Chemistry

    Genomics

    Animal Research

    Cell Biology

    Gene Sequencing

    Systems Biology

    Cancer Diagnostics

    Dna Sequencing

    Oncology

    Life Sciences

    Biomarkers

    Diagnostics

    Molecular Biology

    Research

    Geography

    Asia67%

    North America33%

    Registration Source

    Website Visitors100%

    Job Title

    Student33%

    Educator/Faculty33%

    Medical Laboratory Technician33%

    Organization

    Academic Institution67%

    Manufacturer - Other33%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more