Gene Editing Success: Considerations for maximizing your CRISPR gene editing accuracy and efficiency

CRISPR based gene editing has proven to be an incredibly powerful tool for studying functions of individual proteins, understanding the role of multi-gene pathways, and even knocking out protein function genome-wide for drug candidate screening. With rapidly increasing amounts of information, reagent formats, and downstream applications, there is a lot to consider before beginning a CRISPR experiment.
Key questions to address include: What to consider when choosing a representative biological system? Which reagents are most suitable for your experimental aim? What techniques and equipment will be required for end-point data analysis?

In this webinar we will discuss:

• tips for choosing a cell line/type and the reagent formats best suited for that system
• methods for optimizing reagent delivery
• ways of enriching for edited cells
• techniques for analyzing post-edited cells

Learning objectives:

1. Identify key criteria necessary for determining the ideal cell line and reagent format for your gene editing experiment.

2. Outline techniques and equipment required for end-point data analysis