OCT 03, 2019 12:00 PM PDT

Gene Editing Success: Considerations for maximizing your CRISPR gene editing accuracy and efficiency

Presented at: CRISPR 2019
Sponsored by: Horizon Discovery
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Product Manager, Gene Editing Reagents - Horizon Discovery
    Biography
      Ryan Donnelly received his Master's in Molecular Biotechnology, from George Washington University. He has experience throughout the product marketing life cycle; from uncovering unmet user needs, through product development, and ultimately launch and promotional activities. Ryan has 10 years' experience in life sciences marketing with a heavy focus in molecular biology and related fields. At Horizon Discovery his focus is on the supporting the Edit-R™ gene editing product lines for researchers in the life science community.

    Abstract:

    CRISPR based gene editing has proven to be an incredibly powerful tool for studying functions of individual proteins, understanding the role of multi-gene pathways, and even knocking out protein function genome-wide for drug candidate screening. With rapidly increasing amounts of information, reagent formats, and downstream applications, there is a lot to consider before beginning a CRISPR experiment.
    Key questions to address include: What to consider when choosing a representative biological system? Which reagents are most suitable for your experimental aim? What techniques and equipment will be required for end-point data analysis?


    In this webinar we will discuss:

    • tips for choosing a cell line/type and the reagent formats best suited for that system
    • methods for optimizing reagent delivery
    • ways of enriching for edited cells
    • techniques for analyzing post-edited cells

     

    Learning objectives:

    1. Identify key criteria necessary for determining the ideal cell line and reagent format for your gene editing experiment.

    2. Outline techniques and equipment required for end-point data analysis


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