OCT 03, 2019 12:00 PM PDT

Gene Editing Success: Considerations for maximizing your CRISPR gene editing accuracy and efficiency

Presented at: CRISPR 2019
Sponsored by: Horizon Discovery
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Product Manager, Gene Editing Reagents - Horizon Discovery
    Biography
      Ryan Donnelly received his Master's in Molecular Biotechnology, from George Washington University. He has experience throughout the product marketing life cycle; from uncovering unmet user needs, through product development, and ultimately launch and promotional activities. Ryan has 10 years' experience in life sciences marketing with a heavy focus in molecular biology and related fields. At Horizon Discovery his focus is on the supporting the Edit-R™ gene editing product lines for researchers in the life science community.

    Abstract

    CRISPR based gene editing has proven to be an incredibly powerful tool for studying functions of individual proteins, understanding the role of multi-gene pathways, and even knocking out protein function genome-wide for drug candidate screening. With rapidly increasing amounts of information, reagent formats, and downstream applications, there is a lot to consider before beginning a CRISPR experiment.
    Key questions to address include: What to consider when choosing a representative biological system? Which reagents are most suitable for your experimental aim? What techniques and equipment will be required for end-point data analysis?


    In this webinar we will discuss:

    • tips for choosing a cell line/type and the reagent formats best suited for that system
    • methods for optimizing reagent delivery
    • ways of enriching for edited cells
    • techniques for analyzing post-edited cells

     

    Learning objectives:

    1. Identify key criteria necessary for determining the ideal cell line and reagent format for your gene editing experiment.

    2. Outline techniques and equipment required for end-point data analysis


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