OCT 03, 2019 12:00 PM PDT

Gene Editing Success: Considerations for maximizing your CRISPR gene editing accuracy and efficiency

Presented at: CRISPR 2019
Sponsored by: Horizon Discovery
Speaker

Abstract

CRISPR based gene editing has proven to be an incredibly powerful tool for studying functions of individual proteins, understanding the role of multi-gene pathways, and even knocking out protein function genome-wide for drug candidate screening. With rapidly increasing amounts of information, reagent formats, and downstream applications, there is a lot to consider before beginning a CRISPR experiment.
Key questions to address include: What to consider when choosing a representative biological system? Which reagents are most suitable for your experimental aim? What techniques and equipment will be required for end-point data analysis?


In this webinar we will discuss:

  • tips for choosing a cell line/type and the reagent formats best suited for that system
  • methods for optimizing reagent delivery
  • ways of enriching for edited cells
  • techniques for analyzing post-edited cells

 

Learning objectives:

1. Identify key criteria necessary for determining the ideal cell line and reagent format for your gene editing experiment.

2. Outline techniques and equipment required for end-point data analysis


Show Resources
You May Also Like
JUN 28, 2022 6:45 AM PDT
JUN 28, 2022 6:45 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
MAR 23, 2022 11:00 AM PDT
MAR 23, 2022 11:00 AM PDT
Date: March 23, 2021 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEDT) In this presentation, Dr. Middleton will review the development and deployment of large-scale saliva-based COVID-19 test...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
APR 26, 2022 7:00 AM PDT
C.E. CREDITS
APR 26, 2022 7:00 AM PDT
Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
OCT 03, 2019 12:00 PM PDT

Gene Editing Success: Considerations for maximizing your CRISPR gene editing accuracy and efficiency

Presented at: CRISPR 2019
Sponsored by: Horizon Discovery


Show Resources
Loading Comments...
Show Resources