MAY 09, 2018 9:00 AM PDT

Gene Expression Analysis - Critical Factors for Overcoming Challenges Using qPCR

Sponsored by: QIAGEN
Speakers
  • Director of Global Marketing, Life Science, QIAGEN
    Biography
      Ina is a Director of Global Marketing, Life Science at QIAGEN. She received her PhD from the Max Planck Institute for Biology, studying bacterial pilus adhesion and interaction with the human host receptor/signaling pathway, and publishing a number of scientific publications. At QIAGEN, she is focused on gene expression and gene regulation on a global level and has a keen interest in the emerging field of gene expression studies.
    • Global Product Manager, QIAGEN
      Biography
        Dr. Verena Schramm is a Global Product Manager for RNA sample extraction and Liquid Biopsy at QIAGEN GmbH, Germany. Before taking over these product responsibility, she was a Global Product Manager for NGS library preparation products at QIAGEN.
        Verena holds and M.Sc. in Molecular Biology and a PhD in Bioinformatics. During her PhD at EMBL Heidelberg she studied the impact of copy-number variation during the evolution of non-human primates using Next-Generation Sequencing.
        Before joining QIAGEN, Verena worked as a Sales and Business Development Manager DACH, NL and as a Subject Matter Expert at Sophia Genetics a company focusing on the analysis of clinical NGS data in Diagnostics.

      Abstract

      Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example in protein synthesis (e.g. messenger RNA) or regulation (e.g. microRNA). The accurate quantification of RNAs is key to reliably identifying gene expression profiles and differences between samples. For such complex experiments every step is important. This webinar will guide you through the different steps of gene expression workflows using qPCR, give you a refresher on the technologies behind these workflows, and address how to overcome some common challenges.

      Part 1 focuses on sample purification, including collection, isolation and quality control steps. 

      Part 2 focuses on qPCR assay and analysis, including setup, detection and analysis and interpretation steps. 


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