MAR 31, 2021 9:00 AM PDT

Gene inactivation by CRISPRi: Modulate gene expression with PAM-anchored targeting

Sponsored by: Horizon Discovery
Speaker

Abstract
Date:  March 31, 2021
Time: 9:00am (PDT),  12:00pm (EDT)
 
Knockdown studies are prevalent and powerful ways to illustrate gene function. CRISPRi delivers a new mechanism for gene knockdown, PAM-anchored gene interference without creating double-strand breaks, introducing CRISPR without the cut.
 
CRISPRi harnesses the precision of CRISPR to repress gene function at the transcriptional level. We are proud to introduce the first commercially available complete CRISPRi product line, including synthetic single-guide RNA (sgRNA) and a proprietary repressor construct (dCas9-SALL1-SDS3).
 
CRISPRi-based gene repression relies on a deactivated Cas9 or dCas9 associated with guide RNA. This complex targets a specific DNA sequence and binds to the DNA downstream of a gene's TSS, blocking gene transcription. The functional result is a depletion of the target protein. Our patent-pending repressor fusion, covalently linked to the dCas9 protein, further inhibits target gene expression by recruiting additional proteins involved in chromatin remodeling and gene silencing.
 
CRISPRi is well suited to study any gene and is an ideal platform for simultaneous interrogation of multiple genes. We will share data showing more robust knockdown than demonstrated with existing CRISPRi systems, as well as efficient multiplexing. Importantly, CRISPRi presents another avenue for orthogonal validation studies.
 
Join us for this exciting webinar. We will share information about the development of CRISPRi, experimental strategies for successful implementation, and data on the utility and performance of CRISPRmod.
 
Topics to be covered
  • What is CRISPRi, and how does it work?
  • Tools needed to perform CRISPR gene inactivation
  • Examples, and data from CRISPRi applications
  • Power of CRISPRi in orthogonal validation
 
 
Webinars will be available for unlimited on-demand viewing after live event.

Show Resources
You May Also Like
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
MAR 23, 2022 11:00 AM PDT
MAR 23, 2022 11:00 AM PDT
Date: March 23, 2021 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEDT) In this presentation, Dr. Middleton will review the development and deployment of large-scale saliva-based COVID-19 test...
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
JAN 13, 2022 9:00 AM PST
C.E. CREDITS
JAN 13, 2022 9:00 AM PST
Date: January 13, 2022 Time: 09:00am (PST), 12:00pm (EST) Recently, the Infectious Disease Society of America released guidance on how to approach treatment of infections caused by multidrug...
MAY 19, 2022 8:00 AM PDT
MAY 19, 2022 8:00 AM PDT
Date: May 19, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Mass spectrometry (MS)-based proteomic technologies are increasingly applied in a clinical context for disease classific...
DEC 09, 2021 11:00 AM PST
C.E. CREDITS
DEC 09, 2021 11:00 AM PST
Date: December 09, 2021 Time: 11:00am (PDT), 2:00pm (EDT) The burden of antimicrobial resistance (AMR) has been acknowledged worldwide by leading health institutes. Besides the need for new...
Loading Comments...
Show Resources