MAR 31, 2021 9:00 AM PDT

Gene inactivation by CRISPRi: Modulate gene expression with PAM-anchored targeting

Sponsored by: Horizon Discovery
Speaker

Abstract
Date:  March 31, 2021
Time: 9:00am (PDT),  12:00pm (EDT)
 
Knockdown studies are prevalent and powerful ways to illustrate gene function. CRISPRi delivers a new mechanism for gene knockdown, PAM-anchored gene interference without creating double-strand breaks, introducing CRISPR without the cut.
 
CRISPRi harnesses the precision of CRISPR to repress gene function at the transcriptional level. We are proud to introduce the first commercially available complete CRISPRi product line, including synthetic single-guide RNA (sgRNA) and a proprietary repressor construct (dCas9-SALL1-SDS3).
 
CRISPRi-based gene repression relies on a deactivated Cas9 or dCas9 associated with guide RNA. This complex targets a specific DNA sequence and binds to the DNA downstream of a gene's TSS, blocking gene transcription. The functional result is a depletion of the target protein. Our patent-pending repressor fusion, covalently linked to the dCas9 protein, further inhibits target gene expression by recruiting additional proteins involved in chromatin remodeling and gene silencing.
 
CRISPRi is well suited to study any gene and is an ideal platform for simultaneous interrogation of multiple genes. We will share data showing more robust knockdown than demonstrated with existing CRISPRi systems, as well as efficient multiplexing. Importantly, CRISPRi presents another avenue for orthogonal validation studies.
 
Join us for this exciting webinar. We will share information about the development of CRISPRi, experimental strategies for successful implementation, and data on the utility and performance of CRISPRmod.
 
Topics to be covered
  • What is CRISPRi, and how does it work?
  • Tools needed to perform CRISPR gene inactivation
  • Examples, and data from CRISPRi applications
  • Power of CRISPRi in orthogonal validation
 
 
Webinars will be available for unlimited on-demand viewing after live event.

Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
LIVE NOW!
C.E. CREDITS
LIVE NOW!
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
JUN 08, 2021 9:00 AM PDT
JUN 08, 2021 9:00 AM PDT
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
APR 21, 2021 10:00 AM PDT
C.E. CREDITS
APR 21, 2021 10:00 AM PDT
Date: April 21, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Serological testing for SARS-CoV-2 has been steadily adopted into clinical practice over the course of this pandemic. In this webinar,...
Loading Comments...
Show Resources