JUN 03, 2020 1:00 PM PDT

Gene manipulation in primary human immune cells: Successful strategies and applications for CRISPRko, RNAi and beyond

Sponsored by: Horizon Discovery
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • PhD in Neuroimmunology from the University of Tübingen, Germany, Manager Immunology Screening Services at Horizon Discovery
    Biography
      Verena is a Manager at Horizon Discovery Ltd, based in Cambridge, UK. Horizon Discovery is a world leader in the utilization of gene editing and gene modulation technologies to support the greater understanding of gene function, the genetic drivers behind human disease, and the development of molecular cell and gene therapies for precision medicine. The company's products and services are used by thousands of scientists working in academic institutes, pharmaceutical companies and clinical diagnostic laboratories around the globe. Verena received her doctorate in Neuroimmunology from the University of Tübingen, Germany, and gained a postdoctoral research fellowship from the German Research Foundation that enabled her to intensify her studies on T cell biology in Marc Veldhoen's lab in Cambridge, UK. Before Verena joined Horizon Discovery, she developed assays for hit identification and lead characterisation of multifunctional, oncology-relevant antibody-fragments for the biopharmaceutical company Crescendo Biologics. At Horizon Discovery she is managing a team of scientists, is providing high level scientific expertise and technical leadership for new and ongoing activities within the Immunology services team.

    Abstract

    Gene manipulation studies in primary human immune cells can answer important biological questions within a clinically relevant cellular context. In this webinar we explore the use of several gene editing or modulation tools, including CRISPRko, RNAi and base editing, to modify various primary human immune cell types. In addition, we will discuss numerous applications, such as cell therapy, arrayed and pooled screening, for various gene edited primary immune cell types.

    Learning Objectives:

    1. Understand the usefulness of gene editing and modulation experiments in various primary cell types

    2. Understand the different tools available for gene silencing, knockout and editing

    3. Understand the potential research and therapeutic applications for gene edited cell lines


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