The use of primary cells in vitro is compromised by the limited quantity of cells that can be isolated from one donor, a lack of or very restricted proliferation capacity (e.g. hepatocytes) and/or the change from a quiescent to an activated state (e.g. hepatic stellate cells). The use of immortalized cell lines, on the other hand, is compromised by their transformed, cancerous phenotype. Using the upcyte® technology, primary cells are driven into proliferation using a viral gene transfer system, thus allowing controlled and reversible bypass of cell cycle control mechanisms without inducing immortalization, uncontrolled cell growth or changing the typical phenotype. In this talk liver-specific cells such as upcyte hepatocytes and liver sinusoidal endothelial cells will be presented.
1. Identifying different cell types of the liver and their roles
2. advantages and disadvantages of genetic modification of primary cells