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Identification of a High Titer Lentiviral Production Alternative to Facilitate Regenerative Medicine Therapies

Speaker
  • Development Engineer / Scientist II, CCRM
    Biography
      Calley Hirsch has been a Development Engineer / Scientist II at CCRM in Toronto for 8 months, where she specializes in upstream lentiviral production and gene delivery technologies. Prior to working at CCRM, Calley was a postdoctoral fellow at the Lunenfeld-Tanenbaum Research Institute in Toronto and MD Anderson Cancer Center in Houston working on pluripotent stem cells and somatic cell reprogramming. Calley received her PhD from the University of Saskatchewan.

    Abstract

    Regenerative medicine therapeutics aimed at restoring normal cell function due to trauma and disease are gaining increasing momentum in clinical trials. For this purpose, lentivirus-based gene delivery is actively being investigated to treat a collection of monogenic and neoplastic diseases. The recent success of CAR-T-cell products further indicate an emerging role for lentiviral therapies, however, scalability and cost-effective production still present manufacturing challenges. To circumvent these problems, transient lentivirus production has been proposed in suspension host cells, yet consistent high titer yields remain difficult to achieve. In this presentation, a lentiviral production screen will be discussed where different cell lines, transfection reagents and media have been assessed to define a baseline process for scalable and cost-effective manufacturing of high titer lentivirus.


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    Identification of a High Titer Lentiviral Production Alternative to Facilitate Regenerative Medicine Therapies


    Specialty

    Cancer Research

    Biology

    Medicine

    Biochemistry

    Molecular Biology

    Biomedical Engineering

    Molecular Diagnostics

    Cell Biology

    Geography

    North America50%

    Europe50%

    Registration Source

    Website Visitors100%

    Job Title

    Student50%

    Executive50%

    Organization

    Academic Institution50%

    Other50%


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