DATE: July 20, 2017
TIME: 10:00am PT, 1:00pm ET
Part 1 - Daniel Beacham:
- Case study showing how CyQUANT Direct Cell Proliferation and Fluo-4 calcium imaging assays were used to demonstrate that CultureOne Supplement improves the functional maturation of human neural stem cell derived neurons
- Introduction of next gen HTS ion and voltage sensor imaging tools designed to help investigators phenotype and interrogate excitable cell types in discovery, basic sciences and safety pharmacology research
Part 2 - Nicholas Radio & Michael Derr:
- Review of techniques used to evaluate neurite outgrowth using HCA within a model system of primary cultures of rodent cerebellar granular cells (CGC). As researchers refine neurite outgrowth quantitative analysis using HCA, an emerging question is how to quantify this biology in more complex models that more faithfully recapitulate in vivo environments.
- By improving the maturation of iPSC derived NSC’s to neurons, CultureOne supplement produces neuronal culture characteristics that are ideally suited for High-Content Analysis. Here we demonstrate improved accuracy and simplification of HCA on neuronal cultures differentiated in the presence of CultureOne. Parameters used for phenotypic analysis and characterization of neuronal cultures include neurite outgrowth, neuron count and percent, and progenitor cell quantitation.