JUL 20, 2017 10:00 AM PDT

WEBINAR: Innovative quantitative imaging techniques and their utility in advancing neurobiology research

Speakers
  • Senior Staff Scientist, Discovery Biology, Thermo Fisher Scientific
    Biography
      Dan Beacham is a Senior Staff Scientist in the Discovery Biology group at the Molecular Probes Campus of Thermo Fisher Scientific in Eugene, Oregon. There, he develops cell-based imaging, high throughput screening and biochemical tools for drug discovery and basic sciences research. Dan comes to Thermo Fisher by way of Anchorage Alaska, with an Undergraduate Degree in Chemistry from Willamette University and a PhD in Neurobiology from Oregon Health and Sciences University. He came to Eugene in 2006 following Postdoctorate research at University College London and the Catterall laboratory in the Department of Pharmacology at the University of Washington in Seattle, where he studied molecular mechanisms of ion channel and GPCR control of neural excitability.
    • Manager, Cellular Imaging and Analysis, Thermo Fisher Scientific
      Biography
        Nicholas Radio is a Manager, Cellular Imaging and Analysis for Thermo Fisher Scientific in Pittsburg, PA. He specializes in High Content Analysis, with expertise in drug discovery and in vitro toxicity with emphasis on development of in vitro cardiac, neuronal, and hepatic toxicological assays. Nick obtained his undergraduate degree in Biology from Allegheny College and his Ph.D. in Pharmacology and Toxicology from Duquesne University, where he worked in Dr. Paula Witt-Enderby's laboratory.
      • Scientist, Cell Biology, Thermo Fisher Scientific
        Biography
          Michael Derr's research is focused on development and commercialization of next generation culture systems for pluripotent stem cell culture, differentiation, and neuronal cell culture at Thermo Fisher Scientific on the Cell biology team based in Frederick, MD. With three years of High-Content analysis (HCA) experience, Michael has developed and validated several HCA assays which are routinely used throughout the media development process and have facilitated the successful launch of several cell culture media products. Michael earned his Master's degree from Oregon Health and Science University (OHSU). Prior to starting at Thermo Fisher Scientific in 2014, Michael worked in the lab of Dr. Ron Rosenfeld and Dr. Vivian Hwa at OHSU for five years. His research involved investigating the molecular signaling mechanisms of Growth Hormone and Insulin-like growth factor 1(IGF1) and the role they play in human growth disorders.

        Abstract

        DATE: July 20, 2017
        TIME: 10:00am PT, 1:00pm ET

        Part 1 - Daniel Beacham:

        • Case study showing how CyQUANT Direct Cell Proliferation and Fluo-4 calcium imaging assays were used to demonstrate that CultureOne Supplement improves the functional maturation of human neural stem cell derived neurons
        • Introduction of next gen HTS ion and voltage sensor imaging tools designed to help investigators phenotype and interrogate excitable cell types in discovery, basic sciences and safety pharmacology research
           

        Part 2 - Nicholas Radio & Michael Derr:

        • Review of techniques used to evaluate neurite outgrowth using HCA within a model system of primary cultures of rodent cerebellar granular cells (CGC).  As researchers refine neurite outgrowth quantitative analysis using HCA, an emerging question is how to quantify this biology in more complex models that more faithfully recapitulate in vivo environments.
        • By improving the maturation of iPSC derived NSC’s to neurons, CultureOne supplement produces neuronal culture characteristics that are ideally suited for High-Content Analysis.  Here we demonstrate improved accuracy and simplification of HCA on neuronal cultures differentiated in the presence of CultureOne.  Parameters used for phenotypic analysis and characterization of neuronal cultures include neurite outgrowth, neuron count and percent, and progenitor cell quantitation.
           

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