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Integrated processes for derivation, expansion and differentiation of hiPSC to blood in IPS-Spheres™ cultures

Speaker
  • Associate Staff Scientist, Bioprocessing Technology Institute of Singapore
    Biography
      Dr Alan Lam graduated with a PhD (Biochemistry) from Chinese University of Hong Kong. He is currently the associate staff scientist in the Stem Cell Bioprocessing Group at the Bioprocessing Technology Institute, A*STAR, Singapore. His research interest focuses on developing scalable platforms using microcarriers for adult and pluripotent stem cell derivation, expansion and differentiation. A list of his publications can be found at https://www.a-star.edu.sg/bti/Research/Research-Groups/Stem-Cells.aspx

    Abstract

    Current methods for human induced pluripotent stem cells (hiPSC) derivation, expansion and differentiation can be limited in scalability and costly (due to their labor intensive nature). This can limit their use in cell therapy, drug screening and toxicity assays. One of the approaches that can overcome these limitations is microcarrier (MC) based cultures in which cells are reprogrammed and expanded as cell-MC aggregates and then directly differentiated in the same agitated reactor. This integrated process can be scaled up and eliminate the need for some culture manipulation used in common monolayer and EBs cultures. Here, this presentation describes the principles of using IPS-Spheres™ based integrated hiPSC derivation, expansion and differentiation process. Finally examples of integrated process for generation blood cells as well as challenges to be solved are described. 


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