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Integrated processes for derivation, expansion and differentiation of hiPSC to blood in IPS-Spheres™ cultures

Speaker
  • Associate Staff Scientist, Bioprocessing Technology Institute of Singapore
    BIOGRAPHY

Abstract

Current methods for human induced pluripotent stem cells (hiPSC) derivation, expansion and differentiation can be limited in scalability and costly (due to their labor intensive nature). This can limit their use in cell therapy, drug screening and toxicity assays. One of the approaches that can overcome these limitations is microcarrier (MC) based cultures in which cells are reprogrammed and expanded as cell-MC aggregates and then directly differentiated in the same agitated reactor. This integrated process can be scaled up and eliminate the need for some culture manipulation used in common monolayer and EBs cultures. Here, this presentation describes the principles of using IPS-Spheres™ based integrated hiPSC derivation, expansion and differentiation process. Finally examples of integrated process for generation blood cells as well as challenges to be solved are described. 


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Integrated processes for derivation, expansion and differentiation of hiPSC to blood in IPS-Spheres™ cultures


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