APR 05, 2022 8:00 AM PDT

Investigating antimicrobial mechanisms-of-action using automated genome-wide and gene-specific editing

Sponsored by: Inscripta
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract
Date:  April 05, 2022
Time: 8:00am (PDT),  11:00am (EDT), 5:00pm (CEDT)
 
Members of the cinnamic acid and stilbenoid chemical families are common intermediates in the production of important bioindustrial compounds. However, these molecules can inhibit microbial growth and stifle production capabilities. Since the underlying mechanism of action is unclear, ways to ameliorate inhibition can be time consuming and difficult to identify. Here we use automated, high-throughput, precise and trackable genome editing to probe biological responses to p-coumaric acid and resveratrol. Using a combination of genome-wide and gene-specific edits, we identify genes and individual residues that show enrichment or depletion in the presence of p-coumaric acid or resveratrol. This method represents a new approach to small molecule mechanisms-of-action discovery, providing target leads that can subsequently be used to engineer tolerance or enhance sensitivity to antimicrobial compounds.
 
Learning Objectives
  • Demonstrate a novel approach to mechanism-of-action discovery for small molecule inhibitory compounds 
  • Apply high-throughput, automated, trackable CRISPR-based editing to generate genome-wide perturbations 
  • Investigate the effects of p-coumaric acid and resveratrol on a set of essential genes
  • Uncover genome-wide and gene-specific targets for sensitivity to antimicrobial molecules  
  • Identify potential target leads for engineering tolerance or enhanced sensitivity to inhibitory compounds 
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Show Resources
You May Also Like
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
APR 28, 2022 8:00 AM PDT
APR 28, 2022 8:00 AM PDT
Date: April 28, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Human pluripotent stem cells (PSCs) and their derivatives hold great potentials in...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
FEB 24, 2022 10:00 AM PST
C.E. CREDITS
FEB 24, 2022 10:00 AM PST
Date: February 24, 2021 Time: 10:00am (PST), 1:00pm (EST) One of the largest global public health crises is the rise of antimicrobial-resistant infections. Globally, over 700,000 people die...
Loading Comments...
Show Resources