SEP 23, 2020 10:30 AM PDT

iPSC- and patient- derived intestinal organoids for gastrointestinal research

Presented at: Cell Biology 2020
Sponsored by: MilliporeSigma
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

Intestinal organoids are self-organizing, 3D structures derived from either pluripotent stem cells or from primary tissues with the abiltiy to recapitulate some of the spatial architecture and in vivo function of the organ.  They are increasingly used to model intestinal development and diseases including cancer, celiac disease, inflammatory bowel diseases and host microbiome interactions. In this recent pandemic caused by SARS-CoV-2, studies have shown that this virus can also infect the gut epithelial cells that express ACE2 and TMPRSS2 receptors. Our patient-derived duodenum organoids express both of these markers.  Thus, intestinal organoid models can be a useful tool for researchers studying the basic biology of SARS-CoV-2 and other coronavirses. In this webinar, we describe two model systems for gastrointestinal research: 1) 3dGRO™ Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells that were differentiated in a multi-step process to colon organoids. The organoids express colon-specific markers, can be propagated long-term and functionally swells in response to forskolin. 2) Gastrointestinal Organoid Biobank was developed by researchers at the University of Michigan Center for Gastrointestinal Research (UMCGR). The biobank contains over 50 highly characterized intestinal organoids from both normal and diseased patients derived from multiple regions of the digestive systems including small intestine (duodenum, ileum), stomach, rectum and colon. RNA-Seq data are available for both model systems

Learning Objectives:

1. How to differentiate human iPSC to colon organoids

2. How to culture and maintain intestinal organoids

3. How to use intestinal organoids in a screening or functional assay


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
SEP 29, 2021 11:00 AM PDT
C.E. CREDITS
SEP 29, 2021 11:00 AM PDT
Date: Septembr 29, 2021 Time: 11:00am (PDT), 2:00pm (EDT) 3D cell models are becoming increasingly popular for studying complex biological effects, tissue functionality, and diseases. While...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
Loading Comments...
Show Resources