APR 10, 2019 6:00 AM PDT

Keynote Presentation: Nucleic Acid Detection with CRISPR Diagnostics

C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • MIT McGovern Institute Fellow
    Biography
      Omar Abudayyeh is a MIT McGovern Institute Fellow where he conducts independent research on investigating novel bacterial defense systems for genome editing and gene delivery properties. He previously was at Harvard Medical School and the Harvard-MIT Health Sciences and Technology program as an MD/PhD student. He completed his doctoral work in Feng Zhang's lab at the Broad Institute of MIT and Harvard, where his research centered on novel CRISPR enzymes for applications in genome editing, therapeutics, and diagnostics. Abudayyeh's work focused on trying to uncover novel CRISPR enzymes beyond Cas9 for biotechnological applications. He co-led the discovery and characterization of multiple landmark pieces of work, including the characterization of Cpf1 for novel genome editing applications and the first single-protein RNA-guided RNA-targeting enzyme C2c2/Cas13. His follow-up work on C2c2/Cas13 biology led to the development of SHERLOCK technology, and a new set of tools for precise editing of transcripts and visualizing them in mammalian cells with potential for RNA therapeutics. Abudayyeh graduated from MIT in 2012 with a B.S. in mechanical engineering and biological engineering, where he was a Henry Ford II Scholar and a Barry M. Goldwater Scholar.
    • MIT McGovern Institute Fellow, Massachusetts Institute of Technology
      Biography
        Jonathan Gootenberg earned his bachelor's degree in mathematics and biological engineering at MIT. He received his PhD in Systems Biology from Harvard University, during which he conducted research with Aviv Regev and Feng Zhang at the McGovern Institute and Broad Institute of MIT and Harvard. During his PhD, Gootenberg focused on the development of molecular technologies for treating and sensing disease states, crossing disciplines by utilizing novel computational techniques, microbiology, biochemistry, and molecular biology to uncover new CRISPR tools, including Cas12 and Cas13. He and his co-authors developed Cas13 into a toolbox with uses in fundamental research, therapeutics, and diagnostics. These applications include RNA knockdown, imaging, the base editing platform REPAIR, and the sensitive, specific, and portable diagnostic platform SHERLOCK. He is one of the first members of the McGovern Institute Fellows program, which supports the transition to independent research for exceptional recent PhD graduates, and has received multiple awards, including Forbes 30 Under 30 and Business Insider 30 and Under.

      Abstract

      Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. We combine the RNA- targeting CRISPR effector Cas13 with isothermal amplification to establish a CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch specificity.  We use this Cas13a-based molecular detection platform, termed SHERLOCK, to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify cell-free tumor DNA mutations. By leveraging CRISPR enzymology and lateral flow devices, we demonstrate further improvements on SHERLOCK, including multiplexing and instrument-free readout. CRISPR-Dx forms the basis for multiplexable, portable, rapid, and quantitative detection of nucleic acids.

      Learning Objectives: 

      1. At this seminar, attendees will gain and understanding of CRISPR enzyme diversity and the distinguish characteristics of Cas12 and Cas13 enzymes.
      2. After attending this seminar, attendees will be able to describe the development of CRISPR tools for diagnostics in the field and the clinic


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