SEP 29, 2016 08:00 AM PDT

Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles in Spheroids

Speakers
  • President and CSO at Nano3D Biosciences, Inc. and Adjunct Assistant Professor at University of Texas Health Science Center at Houston
    Biography
      Dr. Glauco R. Souza is the President, CSO, and co-founder of Nano3D Biosciences, Inc. (n3D). He is one of the co-inventors and inventor of all patents related to n3D's 3D cell culturing by magnetic levitation and magnetic 3D bioprinting technologies. Dr. Souza is also Adjunct Assistant Professor at University of Texas Health Science Center at Houston. Prior to co-founding n3D, Dr. Souza was an Odyssey Scholar at UT MD Anderson Cancer Center. During his work at MD Anderson, he applied a multidisciplinary approach to developing nanotechnology tools for tissue targeted imaging, gene delivery, and tissue engineering. Towards the end of his post-doctoral tenure at MD Anderson, in collaboration with Rice University, the genesis of 3D cell culturing by magnetic levitation and magnetic 3D bioprinting took place. Results using these groundbreaking technologies have been reported in various high-impact scientific journals, including Nature Nanotechnology, Nature Protocols, and Nature Reviews Cancer. Now, Dr. Souza's research mission is as CSO of n3D is to advance 3D cell culturing by magnetic levitation and magnetic 3D bioprinting into a routine laboratory tool that will measurably improve the drug discovery process, cancer research, and regenerative medicine. Recently, Glauco was an invited speaker at TEDx Houston, where he shared his thoughts and experience on resonating aspects between science, levitating cells, friendship, and sports as key ingredients for scientific discovery and the entrepreneurial process. Dr. Souza's research has been funded by grants from National Science Foundation (NSF), National Institute of Health (NIH), Department of Defense (DOD), Center for Advancement of Science in Space (CASIS), and Texas Emerging Technology Fund (ETF).

    Abstract:

    PRESENTED BY:



    DATE:
     September 29, 2016
    TIME:  8:00AM PT, 11AM ET

    Interest in 3D cell culture has grown exponentially in recent years, partially due to 3D cultures helping to overcome the challenges of using animal models and inadequacy of 2D cultures.  Challenges are still present because of the physical nature of 3D cultures, especially when using confocal microscopy for visualization as result of limited light penetration through 3D structures. To overcome these limitations, Nano3D Biosciences and  MD Anderson together have  developed methods and protocols using magnetic 3D bioprinting techniques to successfully overcome such challenges.  The results have helped to identify novel approaches to study biology using 3D cell culture and automated confocal imaging of spheroids.
     
    Attendees will learn:
                    -Magnetic 3D bioprinting of spheroids and rings
                    -Tips for high-throughput confocal imaging of 3D cells
                    -Techniques for bioprinting cell co-cultures
                    -Rapid 3D model formation with easy post culture analysis

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