FEB 22, 2017 6:00 AM PST

Mate-pair next generation sequencing as a powerful clinical tool for the characterization of cancer

Speaker

Abstract

Recent advances in DNA sequencing have now made it possible to characterize genomes, transcriptomes and even methylomes which is transforming both basic research and clinical practice. Whole genome sequencing (WGS) is a powerful tool to analyze molecular alterations that occur during the development of cancer, but there are a number of challenges to using WGS as a clinical tool. A practical alternative to WGS is the utilization of mate-pair next generation sequencing (MP-Seq) which can be done for a fraction of the cost of WGS. We have been using MP-Seq to characterize the different roles that human papillomavirus (HPV) plays in the development of different cancers. The cancer that we have been studying is oropharyngeal squamous cell carcinoma (OPSCC) as this cancer is increasingly caused by infection with HPV and there has been an epidemic increase in the incidence of this cancer. We utilized MP-Seq to analyze a group of HPV-positive OPSCCs. What we have found is that HPV is integrated into the genome of OPSCCs in only 30% of these cancers, which is quite distinct from what is observed in cervical cancer. In addition, we have found that the highly unstable common fragile sites and the large genes contained within these regions are hot-spots both for viral integrations and for other alterations in these cancers. Several large common fragile site genes are consistently altered in these cancers. MP-Seq can also determine genome-wide molecular alterations and these alterations can be utilized as cancer-specific markers for the detection of circulating tumor-free DNA from the blood of these patients. This could provide a powerful tool for the development of the liquid biopsy to monitor cancer-treatment of these patients. We will discuss how MP-Seq could thus become a powerful clinical tool for the characterization of genomic alterations in cancer and the development of the liquid biopsy.

Learning Objective 1: Understand how MP-Seq can provide important information about genomic alterations in cancer

Learning Objective 2: Learn about how HPV is involved in the development of different cancers

Learning Objective 3: Learn about how MP-Seq can be a powerful tool to aid in the development of the liquid biopsy

 


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
OCT 27, 2021 6:00 AM PDT
C.E. CREDITS
OCT 27, 2021 6:00 AM PDT
Date: October 27, 2021 Time: 6:00 AM PDT, 9:00 AM EDT Etanercept is a recombinant Fc fusion protein therapeutic that has a complex distribution of post-translation modifications (PTM), such...
FEB 22, 2017 6:00 AM PST

Mate-pair next generation sequencing as a powerful clinical tool for the characterization of cancer



Show Resources
Loading Comments...
Show Resources