APR 28, 2020 10:00 AM PDT

Measuring Single EVs and their Cargo: Sensitive and Specific Vesicle Flow Cytometry (vFCTM)

Speaker
  • Professor at the Scintillon Institute, a non-profit research institute and past director of the National Flow Cytometry Resource at Los Alamos
    BIOGRAPHY

Abstract
DATE:  April 28, 2020
TIME:  10:00am PT
 
All cells release extracellular vesicles (EVs) that can carry molecular cargo to other cells to affect their function. EVs are promising as potential biomarkers, diagnostics and therapeutics, however understanding EV origins, compositional diversity, and biological effects is limited by our ability to measure them. EVs are heterogeneous, small and difficult to measure. Conventional biochemical and molecular methods (Western blot, ELISA, PCR) that report the total signal from target in a sample cannot resolve the identity of specific EVs, while single particle approaches (NTA, RPS, AFM) lack specificity and the ability to measure EV molecular cargo. Flow cytometry is attractive as a single vesicle measurement platform, but conventional instruments and assays lack the sensitivity and specificity to resolve EVs from other small particles and background, leading to artifacts and irreproducible, uninterpretable results. 

A new generation of flow cytometers with high sensitivity photodetectors, together with the use of appropriate calibration protocols, has enabled the development of assays with the sensitivity and specificity to quantitatively and reproducibly measure individual EVs.  Vesicle Flow Cytometry (vFCTM, Cellarcus Biosciences), uses a membrane-selective dye to detect and size individual EVs together with optimized and validated antibodies and staining protocols to measure EV cargo. In this webinar, I will:
 
1) Review the aims and approaches to EV analysis, including challenges for single EV measurement
2) Demonstrate vFCTM using the Beckman Coulter CytoFLEX to count, size, and measure EV cargo
3) Present data on the diversity of EV surface cargo expression, including tetraspanins, integrins, and tumor-associated markers, on individual EVs, and discuss the implications for understanding EV biology
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Show Resources
You May Also Like
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
MAR 23, 2022 11:00 AM PDT
MAR 23, 2022 11:00 AM PDT
Date: March 23, 2021 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEDT) In this presentation, Dr. Middleton will review the development and deployment of large-scale saliva-based COVID-19 test...
FEB 24, 2022 10:00 AM PST
C.E. CREDITS
FEB 24, 2022 10:00 AM PST
Date: February 24, 2021 Time: 10:00am (PST), 1:00pm (EST) One of the largest global public health crises is the rise of antimicrobial-resistant infections. Globally, over 700,000 people die...
Loading Comments...
Show Resources